Toxins 2015, 7(4), 1102-1115; https://doi.org/10.3390/toxins7041102
Alteration in the Expression of Cytochrome P450s (CYP1A1, CYP2E1, and CYP3A11) in the Liver of Mouse Induced by Microcystin-LR
College of Life Science, Henan Normal University, Xinxiang 453007, Henan, China
†
These authors contributed equally to this work.
*
Author to whom correspondence should be addressed.
Academic Editor: Luis M. Botana
Received: 26 February 2015 / Revised: 23 March 2015 / Accepted: 24 March 2015 / Published: 30 March 2015
(This article belongs to the Collection Marine and Freshwater Toxins)
Abstract
Microcystins (MCs) are cyclic heptapeptide toxins and can accumulate in the liver. Cytochrome P450s (CYPs) play an important role in the biotransformation of endogenous substances and xenobiotics in animals. It is unclear if the CYPs are affected by MCs exposure. The objective of this study was to evaluate the effects of microcystin-LR (MCLR) on cytochrome P450 isozymes (CYP1A1, CYP2E1, and CYP3A11) at mRNA level, protein content, and enzyme activity in the liver of mice the received daily, intraperitoneally, 2, 4, and 8 µg/kg body weight of MCLR for seven days. The result showed that MCLR significantly decreased ethoxyresorufin-O-deethylase (EROD) (CYP1A1) and erythromycin N-demthylase (ERND) (CYP3A11) activities and increased aniline hydroxylase (ANH) activity (CYP2E1) in the liver of mice during the period of exposure. Our findings suggest that MCLR exposure may disrupt the function of CYPs in liver, which may be partly attributed to the toxicity of MCLR in mice. View Full-TextKeywords:
MCLR; mouse; cytochrome P450; mRNA level; protein content; enzyme activity
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Zhang, B.; Liu, Y.; Li, X. Alteration in the Expression of Cytochrome P450s (CYP1A1, CYP2E1, and CYP3A11) in the Liver of Mouse Induced by Microcystin-LR. Toxins 2015, 7, 1102-1115.