Next Article in Journal / Special Issue
Isolation and Characterization of Cylindrospermopsis raciborskii Strains from Finished Drinking Water
Previous Article in Journal
An Inducible Cre-lox System to Analyze the Role of LLO in Listeria monocytogenes Pathogenesis
Previous Article in Special Issue
Suppressing Cyanobacteria with Hydrogen Peroxide Is More Effective at High Light Intensities
Open AccessArticle

Morphological and Molecular Identification of Microcystin-Producing Cyanobacteria in Nine Shallow Bulgarian Water Bodies

1
AgroBio Institute, Bulgarian Agricultural Academy, BG-1164 Sofia, Bulgaria
2
Faculty of Biology, Department of Botany, Sofia University, BG-1164 Sofia, Bulgaria
3
Institute of Botany, Innsbruck University, A-6020 Innsbruck, Austria
*
Author to whom correspondence should be addressed.
Toxins 2020, 12(1), 39; https://doi.org/10.3390/toxins12010039
Received: 6 November 2019 / Revised: 3 January 2020 / Accepted: 6 January 2020 / Published: 8 January 2020
The paper presents results from the first application of polyphasic approach in studies of field samples from Bulgaria. This approach, which combined the conventional light microscopy (LM) and molecular-genetic methods (based on PCR amplified fragments of microcystin synthetase gene mcyE), revealed that almost all microcystin-producers in the studied eutrophic waterbodies belong to the genus Microcystis. During the molecular identification of toxin-producing strains by use of HEPF × HEPR pair of primers, we obtained 57 sequences, 56 of which formed 28 strains of Microcystis, spread in six clusters of the phylogenetic tree. By LM, seven Microcystis morphospecies were identified (M. aeruginosa, M. botrys, M. flos-aquae, M. natans, M. novacekii, M. smithii, and M. wesenbergii). They showed significant morphological variability and contributed from <1% to 98% to the total biomass. All data support the earlier opinions that taxonomic revision of Microcystis is needed, proved the presence of toxigenic strains in M. aeruginosa and M. wesenbergii, and suppose their existence in M. natans. Our results demonstrated also that genetic sequencing, and the use of HEPF × HEPR pair in particular, can efficiently serve in water quality monitoring for identifying the potential risk from microcystins, even in cases of low amounts of Microcystis in the water.
Keywords: cyanoprokaryotes; cyanotoxins; nodularins; phytoplankton; polyphasic approach cyanoprokaryotes; cyanotoxins; nodularins; phytoplankton; polyphasic approach
MDPI and ACS Style

Radkova, M.; Stefanova, K.; Uzunov, B.; Gärtner, G.; Stoyneva-Gärtner, M. Morphological and Molecular Identification of Microcystin-Producing Cyanobacteria in Nine Shallow Bulgarian Water Bodies. Toxins 2020, 12, 39.

Show more citation formats Show less citations formats
Note that from the first issue of 2016, MDPI journals use article numbers instead of page numbers. See further details here.

Article Access Map by Country/Region

1
Back to TopTop