Next Article in Journal
Calcium-Induced Activity and Folding of a Repeat in Toxin Lipase from Antarctic Pseudomonas fluorescens Strain AMS8
Previous Article in Journal
Phytotoxic Responses of Soybean (Glycine max L.) to Botryodiplodin, a Toxin Produced by the Charcoal Rot Disease Fungus, Macrophomina phaseolina
Open AccessArticle

Revisiting Old Ionophore Lasalocid as a Novel Inhibitor of Multiple Toxins

Service d’Ingénierie Moléculaire des Protéines (SIMOPRO), CEA, Université Paris-Saclay, F-91191 Gif-sur-Yvette, France
Service de Chimie Bio-organique et Marquage (SCBM), CEA, Université Paris-Saclay, F-91191 Gif-sur-Yvette, France
Unité des Toxines Bactériennes, Institut Pasteur, ERL 6002, F-75015 Paris, France
Authors to whom correspondence should be addressed.
Toxins 2020, 12(1), 26;
Received: 16 October 2019 / Revised: 9 December 2019 / Accepted: 14 December 2019 / Published: 1 January 2020
(This article belongs to the Special Issue Inhibitors and Countermeasures against Bacterial and Plant Toxins)
The ionophore lasalocid is widely used as a veterinary drug against coccidiosis. We found recently that lasalocid protects cells from two unrelated bacterial toxins, the cytotoxic necrotizing factor-1 (CNF1) from Escherichia. coli and diphtheria toxin. We evaluated lasalocid’s capacity to protect cells against other toxins of medical interest comprising toxin B from Clostridium difficile, Shiga-like toxin 1 from enterohemorrhagic E. coli and exotoxin A from Pseudomonas aeruginosa. We further characterized the impact of lasalocid on the endolysosomal and the retrograde pathways and organelle integrity, especially the Golgi apparatus. We found that lasalocid protects cells from all toxins tested and impairs the drop of vesicular pH along the trafficking pathways that are required for toxin sorting and translocation to the cytoplasm. Lasalocid also has an impact on the cellular distribution of GOLPH4 and GOLPH2 Golgi markers. Other intracellular trafficking compartments positive for EEA1 and Rab9A display a modified cellular pattern. In conclusion, lasalocid protects cells from multiple deadly bacterial toxins by corrupting vesicular trafficking and Golgi stack homeostasis. View Full-Text
Keywords: lasalocid; toxin; Golgi lasalocid; toxin; Golgi
Show Figures

Figure 1

MDPI and ACS Style

Mahtal, N.; Wu, Y.; Cintrat, J.-C.; Barbier, J.; Lemichez, E.; Gillet, D. Revisiting Old Ionophore Lasalocid as a Novel Inhibitor of Multiple Toxins. Toxins 2020, 12, 26.

Show more citation formats Show less citations formats
Note that from the first issue of 2016, MDPI journals use article numbers instead of page numbers. See further details here.

Article Access Map by Country/Region

Back to TopTop