Next Article in Journal / Special Issue
Consumption of Dairy Yogurt Containing Lactobacillus paracasei ssp. paracasei, Bifidobacterium animalis ssp. lactis and Heat-Treated Lactobacillus plantarum Improves Immune Function Including Natural Killer Cell Activity
Previous Article in Journal
Blackcurrant Alters Physiological Responses and Femoral Artery Diameter during Sustained Isometric Contraction
Previous Article in Special Issue
Effect of Probiotics on Metabolic Outcomes in Pregnant Women with Gestational Diabetes: A Systematic Review and Meta-Analysis of Randomized Controlled Trials
Open AccessArticle

Lactobacillus reuteri I5007 Modulates Intestinal Host Defense Peptide Expression in the Model of IPEC-J2 Cells and Neonatal Piglets

1
State Key Laboratory of Animal Nutrition, China Agricultural University, Beijing, 100193, China
2
Institute of Food Science and Technology CAAS, Chinese Academy of Agricultural Sciences, Beijing, 100193, China
3
Department of Animal and Poultry Science, University of Saskatchewan, SK S7N 5C5, Canada
4
Department of Animal Science, Oklahoma State University, Stillwater, OK 74074, USA
*
Author to whom correspondence should be addressed.
These authors contributed equally to this work.
Nutrients 2017, 9(6), 559; https://doi.org/10.3390/nu9060559
Received: 22 April 2017 / Revised: 25 May 2017 / Accepted: 26 May 2017 / Published: 31 May 2017
(This article belongs to the Special Issue Prebiotics and Probiotics)
Modulation of the synthesis of endogenous host defense peptides (HDPs) by probiotics represents a novel antimicrobial approach for disease control and prevention, particularly against antibiotic-resistant infections in human and animals. However, the extent of HDP modulation by probiotics is species dependent and strain specific. In the present study, The porcine small intestinal epithelial cell line (IPEC-J2) cells and neonatal piglets were used as in-vitro and in-vivo models to test whether Lactobacillus reuteri I5007 could modulate intestinal HDP expression. Gene expressions of HDPs, toll-like receptors, and fatty acid receptors were determined, as well as colonic short chain fatty acid concentrations and microbiota. Exposure to 108 colony forming units (CFU)/mL of L. reuteri I5007 for 6 h significantly increased the expression of porcine β-Defensin2 (PBD2), pBD3, pBD114, pBD129, and protegrins (PG) 1-5 in IPEC-J2 cells. Similarly, L. reuteri I5007 administration significantly increased the expression of jejunal pBD2 as well as colonic pBD2, pBD3, pBD114, and pBD129 in neonatal piglets (p < 0.05). This was probably associated with the increase in colonic butyric acid concentration and up-regulating expression of Peroxisome Proliferator-Activated Receptor-γ (PPAR-γ) and G Protein-Coupled Receptor 41 (GPR41) (p < 0.05), but not with stimulation of Pattern-Recognition Receptors. Additionally, supplementation with L. reuteri I5007 in the piglets did not affect the colonic microbiota structure. Our findings suggested that L. reuteri I5007 could modulate intestinal HDP expression and improve the gut health of neonatal piglets, probably through the increase in colonic butyric acid concentration and the up-regulation of the downstream molecules of butyric acid, PPAR-γ and GPR41, but not through modifying gut microbiota structure. View Full-Text
Keywords: Lactobacillus reuteri; intestinal epithelial cells; neonatal piglets; host defense peptide; gut microbiota Lactobacillus reuteri; intestinal epithelial cells; neonatal piglets; host defense peptide; gut microbiota
Show Figures

Figure 1

MDPI and ACS Style

Liu, H.; Hou, C.; Wang, G.; Jia, H.; Yu, H.; Zeng, X.; Thacker, P.A.; Zhang, G.; Qiao, S. Lactobacillus reuteri I5007 Modulates Intestinal Host Defense Peptide Expression in the Model of IPEC-J2 Cells and Neonatal Piglets. Nutrients 2017, 9, 559.

Show more citation formats Show less citations formats
Note that from the first issue of 2016, MDPI journals use article numbers instead of page numbers. See further details here.

Article Access Map

1
Back to TopTop