Next Article in Journal
Taste Receptors in Upper Airway Innate Immunity
Previous Article in Journal
Multiple Dimensions of Sweet Taste Perception Altered after Sleep Curtailment
Article

Gene Expression and Cardiometabolic Phenotypes of Vitamin D-Deficient Overweight and Obese Black Children

1
Department of Pediatrics, UPMC Children’s Hospital of Pittsburgh, University of Pittsburgh, Pittsburgh, PA 15224, USA
2
Division of Pediatric Pulmonary Medicine, UPMC Children’s Hospital of Pittsburgh, University of Pittsburgh, Pittsburgh, PA 15224, USA
3
Department of Human Genetics, University of Pittsburgh, Pittsburgh, PA 15261, USA
*
Author to whom correspondence should be addressed.
Nutrients 2019, 11(9), 2016; https://doi.org/10.3390/nu11092016
Received: 31 July 2019 / Revised: 13 August 2019 / Accepted: 16 August 2019 / Published: 28 August 2019
Associations between whole blood transcriptome and clinical phenotypes in vitamin D-deficient overweight and obese children can provide insight into the biological effects of vitamin D and obesity. We determined differentially expressed genes (DEGs) in relation to body mass index (BMI) in vitamin D-deficient black children with a BMI ≥ 85th percentile and ascertained the cardiometabolic phenotypes associated with the DEGs. We examined whole-blood transcriptome gene expression by RNA sequencing and cardiometabolic profiling in 41, 10- to 18-year-old children. We found 296 DEGs in association with BMI after adjusting for age, race, sex, and pubertal status. Cardiometabolic phenotypes associated with the BMI-related DEGs, after adjusting for age, sex, pubertal status, and %total body fat, were (i) flow-mediated dilation (marker of endothelial function), (ii) c-reactive protein (marker of inflammation), and (iii) leptin (adipocytokine). Canonical pathways of relevance for childhood obesity and its phenotypes that were significantly associated with the BMI-related DEGs affected immune cell function/inflammation, vascular health, metabolic function, and cell survival/death; several immune and inflammatory pathways overlapped across the three phenotypes. We have identified transcriptome-based biomarkers associated with BMI in vitamin D-deficient, overweight and obese black children. Modulating effects of vitamin D supplementation on these biomarkers and their related phenotypes need further exploration. View Full-Text
Keywords: c-reactive protein; gene expression; leptin; endothelium; vitamin D deficiency c-reactive protein; gene expression; leptin; endothelium; vitamin D deficiency
Show Figures

Figure 1

MDPI and ACS Style

Rajakumar, K.; Yan, Q.; Khalid, A.T.; Feingold, E.; Vallejo, A.N.; Demirci, F.Y.; Kamboh, M.I. Gene Expression and Cardiometabolic Phenotypes of Vitamin D-Deficient Overweight and Obese Black Children. Nutrients 2019, 11, 2016. https://doi.org/10.3390/nu11092016

AMA Style

Rajakumar K, Yan Q, Khalid AT, Feingold E, Vallejo AN, Demirci FY, Kamboh MI. Gene Expression and Cardiometabolic Phenotypes of Vitamin D-Deficient Overweight and Obese Black Children. Nutrients. 2019; 11(9):2016. https://doi.org/10.3390/nu11092016

Chicago/Turabian Style

Rajakumar, Kumaravel, Qi Yan, Arshad T. Khalid, Eleanor Feingold, Abbe N. Vallejo, F. Y. Demirci, and M. I. Kamboh 2019. "Gene Expression and Cardiometabolic Phenotypes of Vitamin D-Deficient Overweight and Obese Black Children" Nutrients 11, no. 9: 2016. https://doi.org/10.3390/nu11092016

Find Other Styles
Note that from the first issue of 2016, MDPI journals use article numbers instead of page numbers. See further details here.

Article Access Map by Country/Region

1
Back to TopTop