Next Article in Journal
Nutrition and Risk of Stroke
Previous Article in Journal
Optimal Time and Target for Evaluating Energy Delivery after Adjuvant Feeding with Small Bowel Enteral Nutrition in Critically Ill Patients at High Nutrition Risk
Open AccessArticle

Homocysteine Metabolism in Children and Adolescents: Influence of Age on Plasma Biomarkers and Correspondent Genotype Interactions

1
Faculty of Life Sciences, University of Madeira, Campus da Penteada, 9000-390 Funchal, Portugal
2
Centro de Química da Madeira, University of Madeira, Campus da Penteada, 9000-390 Funchal, Portugal
3
Metabolism and Genetics Laboratory, Research Institute for Medicines (i.Med.ULisboa), Faculty of Pharmacy, University of Lisbon, 1649-003 Lisbon, Portugal
4
Department of Biochemistry and Human Biology, Faculty of Pharmacy, University of Lisbon, 1649-003 Lisbon, Portugal
5
Department of Nutritional Sciences, The Pennsylvania State University, University Park, PA 16802, USA
*
Author to whom correspondence should be addressed.
Nutrients 2019, 11(3), 646; https://doi.org/10.3390/nu11030646
Received: 28 January 2019 / Revised: 26 February 2019 / Accepted: 6 March 2019 / Published: 16 March 2019
Background: Imbalance of homocysteine (Hcy) metabolism links with several pathologies; nevertheless, it is poorly characterized in pediatric populations. This study investigated the impact of age on plasma concentrations of Hcy and relevant biomarkers along with correspondent genotype interactions. Methods: A healthy pediatric cohort aged 9 (n = 195) and 17 (n = 128) years old (yo) was studied. Immunoassays and GC-MS-SIM-mode quantified plasma levels of Hcy and biomarkers. PCR-RFLP or quantitative-PCR assays assessed common variations in related genes. Results: Age impacted on levels of Hcy and metabolic markers: older children presented with the lowest folates and total-cobalamin (tCbl), while with the highest Hcy concentrations, whereas methylmalonic acid (MMA) and holotranscobalamin (Holo-TC) levels remained similar in 9-yo and 17-yo children. The relationships between B-vitamins and metabolic markers were also dependent on age. Only in the older children, MMA correlated with tCbl and Holo-TC, and MMA levels were markedly higher in the 17-yo subjects presenting with the lowest quartiles of Holo-TC concentrations. Lastly, age also impacted on the correlations between genotype and biomarkers. In the 17-yo group, however not in the 9-yo children, tHcy differed between MTHFR 677 genotypes, with subjects who had the MTHFR 677TT genotype displaying the highest tHcy concentrations. Conclusions: Age impacts on the Hcy metabolism dynamics in a pediatric population. View Full-Text
Keywords: vitamin B12; methylmalonic acid; folate; homocysteine; MTHFR vitamin B12; methylmalonic acid; folate; homocysteine; MTHFR
Show Figures

Figure 1

MDPI and ACS Style

Caldeira-Araújo, H.; Ramos, R.; Florindo, C.; Rivera, I.; Castro, R.; Tavares de Almeida, I. Homocysteine Metabolism in Children and Adolescents: Influence of Age on Plasma Biomarkers and Correspondent Genotype Interactions. Nutrients 2019, 11, 646.

Show more citation formats Show less citations formats
Note that from the first issue of 2016, MDPI journals use article numbers instead of page numbers. See further details here.

Article Access Map by Country/Region

1
Search more from Scilit
 
Search
Back to TopTop