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Nutrients 2018, 10(4), 514; https://doi.org/10.3390/nu10040514

Prophylactic Neuroprotection of Total Glucosides of Paeoniae Radix Alba against Semen Strychni-Induced Neurotoxicity in Rats: Suppressing Oxidative Stress and Reducing the Absorption of Toxic Components

1
School of Pharmacy, Shenyang Pharmaceutical University, Shenyang Pharmaceutical University, No. 103, Wenhua Road, Shenyang 110000, China
2
School of Traditional Chinese Material Medica, Shenyang Pharmaceutical University, No. 103, Wenhua Road, Shenyang 110000, China
3
Southern Research Institute, 2000 9th Ave. s., Birmingham 35205, AL, USA
*
Author to whom correspondence should be addressed.
Received: 7 February 2018 / Revised: 8 April 2018 / Accepted: 13 April 2018 / Published: 20 April 2018
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Abstract

Strychnos alkaloids (SAs) are the main toxic constituents in Semen Strychni, a traditional Chinese medicine, which is known for its fatal neurotoxicity. Hence, the present study was carried out to evaluate the neurotoxicity induced by SAs and the pre-protective effects of the total glucosides of Paeoniae Radix Alba (TGP). An SA brain damage model was firstly established. The neurotoxicity induced by SAs and the pre-protective effects of TGP were confirmed by physical and behavioral testing, biochemical assay, and histological examination. Then, a liquid chromatography-tandem mass spectrometry method was developed and validated to investigate the time-course change and distribution of strychnine and brucine (two main SAs) in the brain after oral SA administration with or without TGP pretreatment. Biochemical analysis results indicated that TGP could ameliorate the oxidative stress status caused by SAs. Time-course change and distribution studies demonstrated that strychnine and brucine were rapidly absorbed into the brain, peaked early at 0.5 h, and were mainly located in the hippocampus and cerebellum. TGP showed a pre-protective effect against neurotoxicity by reducing the absorption of toxic alkaloids into the brain. These findings could provide beneficial information in facilitating future studies of Semen Strychni neurotoxicity and developing herbal medicines to alleviate neurotoxicity in the clinic. View Full-Text
Keywords: Semen Strychni; neurotoxicity; Paeoniae Radix Alba; neuro-protective effects Semen Strychni; neurotoxicity; Paeoniae Radix Alba; neuro-protective effects
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Li, S.; Chu, Y.; Zhang, R.; Sun, L.; Chen, X. Prophylactic Neuroprotection of Total Glucosides of Paeoniae Radix Alba against Semen Strychni-Induced Neurotoxicity in Rats: Suppressing Oxidative Stress and Reducing the Absorption of Toxic Components. Nutrients 2018, 10, 514.

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