Curcumin Inhibits Age-Related Vascular Changes in Aged Mice Fed a High-Fat Diet
Abstract
:1. Introduction
2. Materials and Methods
2.1. Reagent
2.2. Animals
2.3. Superoxide Production
2.4. Measurement of Urinary 8-OHdG
2.5. Measurements of mRNA
2.6. Western Blot Analysis
2.7. SA β-Gal Staining
2.8. Bilirubin Staining
2.9. Serum Biochemical Analysis
2.10. Measurement of MCP-1 Levels in Blood
2.11. Statistical Test
3. Results
3.1. Curcumin Controls Weight Gain and Increases the Blood Glucose Level and Blood Cholesterol Level Associated with Long-Term Administration HFD
3.2. Curcumin-Mixed Feed Enhances HO-1 Enzyme Activity
3.3. Curcumin-Mixed Feed Suppresses Vascular and Systemic Oxidative Stress
3.4. Enhanced HO-1 Activity Due to Curcumin-Mixed Feed Maintains Sirt1 Expression
3.5. Curcumin-Mixed Feed Inhibits Chronic Inflammation Associated with Vascular Aging
4. Discussion
5. Conclusions
Author Contributions
Funding
Acknowledgments
Conflicts of Interest
References
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Parameters | y-CTL | MF | HFD | HFD + Cu | HFD + Cu + SnMP |
---|---|---|---|---|---|
Food intake (g/day) | 4.2 ± 0.23 | 4.3 ± 0.23 | 4.2 ± 0.20 | 3.7 ± 0.13 § | |
Body weight (g) | 41.8 ± 1.12 † (n = 18) | 50.8 ± 1.80 (n = 14) | 38.4 ± 1.20 † (n = 18) | 31.3 ± 0.74 †§ (n = 12) | |
Systolic blood pressure (mmHg) | 110 ± 4.3 | 113 ± 2.0 | 111 ± 42.6 | 106 ± 3.3 | 103 ± 8.7 |
Blood glucose (mg/dL) | 119 ± 5.3 | 127 ± 4.2 | 211 ± 16.8 * | 139 ± 6.0 *† | 155 ± 8.6 *†§ |
Total cholesterol (mg/dL) | 81 ± 7.1 | 91 ± 4.8 | 123 ± 11.9 * | 99 ± 8.1 *† | 103 ± 8.6 * |
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Takano, K.; Tatebe, J.; Washizawa, N.; Morita, T. Curcumin Inhibits Age-Related Vascular Changes in Aged Mice Fed a High-Fat Diet. Nutrients 2018, 10, 1476. https://doi.org/10.3390/nu10101476
Takano K, Tatebe J, Washizawa N, Morita T. Curcumin Inhibits Age-Related Vascular Changes in Aged Mice Fed a High-Fat Diet. Nutrients. 2018; 10(10):1476. https://doi.org/10.3390/nu10101476
Chicago/Turabian StyleTakano, Kenichiro, Junko Tatebe, Naohiro Washizawa, and Toshisuke Morita. 2018. "Curcumin Inhibits Age-Related Vascular Changes in Aged Mice Fed a High-Fat Diet" Nutrients 10, no. 10: 1476. https://doi.org/10.3390/nu10101476