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Open AccessArticle

Facile Bioinspired Preparation of [email protected] Hydroxyapatite Nanoflowers for the Biosynthesis of 5′-Fluorodeoxy Adenosine

1
College of Materials, Chemistry and Chemical Engineering, Hangzhou Normal University, Hangzhou 311121, China
2
Holistic Integrative Pharmacy Institutes, College of Medicine, Hangzhou Normal University, Hangzhou 311121, China
*
Authors to whom correspondence should be addressed.
Sustainability 2020, 12(1), 431; https://doi.org/10.3390/su12010431
Received: 22 November 2019 / Revised: 17 December 2019 / Accepted: 18 December 2019 / Published: 6 January 2020
(This article belongs to the Special Issue Synthesis Methods with Green Chemistry Aspect)
To develop an environmentally friendly biocatalyst for the efficient synthesis of organofluorine compounds, we prepared the [email protected] hydroxyapatite nanoflowers (FHAp-NFs) using fluorinase expressed in Escherichia coli Rosetta (DE3) as the biomineralization framework. The obtained [email protected] were characterized by scanning electron microscope (SEM), X-ray diffraction (XRD), and FT-IR spectrum and used in the enzymatic synthesis of 5′-fluorodeoxy adenosin with S-adenosyl-L-methionine and fluoride as substrate. At an optimum pH of 7.5, fluorinase confined in the hybrid nanoflowers presents an approximately 2-fold higher synthetic activity than free fluorinase. Additionally, after heating at 30 °C for 8 h, the FHAp-NFs retained approximately 80.0% of the initial activity. However, free enzyme could remain only 48.2% of its initial activity. The results indicate that the fluoride and hybrid nanoflowers efficiently enhance the catalytic activity and thermal stability of fluorinase in the synthesis of 5′-fluorodeoxy adenosine, which gives a green method for producing the fluorinated organic compounds. View Full-Text
Keywords: organofluorine compound; fluorinase; 5′-fluorodeoxy adenosine; biomineralization; immobilization; fluoridated hydroxyapatite nanoflowers organofluorine compound; fluorinase; 5′-fluorodeoxy adenosine; biomineralization; immobilization; fluoridated hydroxyapatite nanoflowers
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MDPI and ACS Style

Li, N.; Hu, B.; Wang, A.; Li, H.; Yin, Y.; Mao, T.; Xie, T. Facile Bioinspired Preparation of [email protected] Hydroxyapatite Nanoflowers for the Biosynthesis of 5′-Fluorodeoxy Adenosine. Sustainability 2020, 12, 431. https://doi.org/10.3390/su12010431

AMA Style

Li N, Hu B, Wang A, Li H, Yin Y, Mao T, Xie T. Facile Bioinspired Preparation of [email protected] Hydroxyapatite Nanoflowers for the Biosynthesis of 5′-Fluorodeoxy Adenosine. Sustainability. 2020; 12(1):431. https://doi.org/10.3390/su12010431

Chicago/Turabian Style

Li, Ningning; Hu, Bingjing; Wang, Anming; Li, Huimin; Yin, Youcheng; Mao, Tianyu; Xie, Tian. 2020. "Facile Bioinspired Preparation of [email protected] Hydroxyapatite Nanoflowers for the Biosynthesis of 5′-Fluorodeoxy Adenosine" Sustainability 12, no. 1: 431. https://doi.org/10.3390/su12010431

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