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International Journal of Plant Biology is published by MDPI from Volume 13 Issue 1 (2022). Previous articles were published by another publisher in Open Access under a CC-BY (or CC-BY-NC-ND) licence, and they are hosted by MDPI on mdpi.com as a courtesy and upon agreement with PAGEPress.
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Article

Proteomic Analysis of the Pulvinus, a Heliotropic Tissue, in Glycine max

by
Hakme Lee
1,
Wesley M. Garrett
2,
Joseph Sullivan
1,
Irwin Forseth
1 and
Savithiry S. Natarajan
3,*
1
Department of Plant Sciences and Landscape Architecture, University of Maryland, College Park, MD 20742, USA
2
Animal Bioscience and Biotechnology Laboratory, USDA-ARS, Beltsville, MD 20705, USA
3
Soybean Genomics and Improvement Laboratory, USDA-ARS, Beltsville, MD 20705, USA
*
Author to whom correspondence should be addressed.
Int. J. Plant Biol. 2014, 5(1), 4887; https://doi.org/10.4081/pb.2014.4887
Submission received: 27 May 2013 / Revised: 19 August 2013 / Accepted: 23 April 2014 / Published: 23 June 2014
Versions Notes

Abstract

Certain plant species respond to light, dark, and other environmental factors by leaf movement. Leguminous plants both track and avoid the sun through turgor changes of the pulvinus tissue at the base of leaves. Mechanisms leading to pulvinar turgor flux, particularly knowledge of the proteins involved, are not well-known. In this study we used two-dimensional gel electrophoresis and liquid chromatography-tandom mass spectrometry to separate and identify the proteins located in the soybean pulvinus. A total of 183 spots were separated and 195 proteins from 165 spots were identified and functionally analyzed using single enrichment analysis for gene ontology terms. The most significant terms were related to proton transport. Comparison with guard cell proteomes revealed similar significant processes but a greater number of pulvinus proteins are required for comparable analysis. To our knowledge, this is a novel report on the analysis of proteins found in soybean pulvinus. These findings provide a better understanding of the proteins required for turgor change in the pulvinus.
Keywords: soybean; heliotropism; nyctinasty; proteomics; LC-MS/MS soybean; heliotropism; nyctinasty; proteomics; LC-MS/MS

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MDPI and ACS Style

Lee, H.; Garrett, W.M.; Sullivan, J.; Forseth, I.; Natarajan, S.S. Proteomic Analysis of the Pulvinus, a Heliotropic Tissue, in Glycine max. Int. J. Plant Biol. 2014, 5, 4887. https://doi.org/10.4081/pb.2014.4887

AMA Style

Lee H, Garrett WM, Sullivan J, Forseth I, Natarajan SS. Proteomic Analysis of the Pulvinus, a Heliotropic Tissue, in Glycine max. International Journal of Plant Biology. 2014; 5(1):4887. https://doi.org/10.4081/pb.2014.4887

Chicago/Turabian Style

Lee, Hakme, Wesley M. Garrett, Joseph Sullivan, Irwin Forseth, and Savithiry S. Natarajan. 2014. "Proteomic Analysis of the Pulvinus, a Heliotropic Tissue, in Glycine max" International Journal of Plant Biology 5, no. 1: 4887. https://doi.org/10.4081/pb.2014.4887

APA Style

Lee, H., Garrett, W. M., Sullivan, J., Forseth, I., & Natarajan, S. S. (2014). Proteomic Analysis of the Pulvinus, a Heliotropic Tissue, in Glycine max. International Journal of Plant Biology, 5(1), 4887. https://doi.org/10.4081/pb.2014.4887

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