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Article

Effects of Surface Protein Adsorption on the Distribution and Retention of Intratumorally Administered Gold Nanoparticles

1
Department of Nanomedicine, Houston Methodist Research Institute, Houston, TX 77030, USA
2
Department of Electronics, Politecnico di Torino, 10129 Torino, Italy
3
Department of Physics & Astronomy, Rice University, Houston, TX 77005, USA
4
Department of Radiation Oncology, Houston Methodist Research Institute, Houston, TX 77030, USA
5
Department of Chemistry, Rice University, Houston, TX 77005, USA
6
Department of Surgery, Houston Methodist Research Institute, Houston, TX 77030, USA
7
Department of Cardiovascular Surgery, Houston Methodist Research Institute, Houston, TX 77030, USA
*
Author to whom correspondence should be addressed.
Academic Editors: Kytai T. Nguyen and Jyothi U. Menon
Pharmaceutics 2021, 13(2), 216; https://doi.org/10.3390/pharmaceutics13020216
Received: 23 December 2020 / Revised: 26 January 2021 / Accepted: 29 January 2021 / Published: 5 February 2021
The heterogeneous distribution of delivery or treatment modalities within the tumor mass is a crucial limiting factor for a vast range of theranostic applications. Understanding the interactions between a nanomaterial and the tumor microenvironment will help to overcome challenges associated with tumor heterogeneity, as well as the clinical translation of nanotheranostic materials. This study aims to evaluate the influence of protein surface adsorption on gold nanoparticle (GNP) biodistribution using high-resolution computed tomography (CT) preclinical imaging in C57BL/6 mice harboring Lewis lung carcinoma (LLC) tumors. LLC provides a valuable model for study due to its highly heterogenous nature, which makes drug delivery to the tumor challenging. By controlling the adsorption of proteins on the GNP surface, we hypothesize that we can influence the intratumoral distribution pattern and particle retention. We performed an in vitro study to evaluate the uptake of GNPs by LLC cells and an in vivo study to assess and quantify the GNP biodistribution by injecting concentrated GNPs citrate-stabilized or passivated with bovine serum albumin (BSA) intratumorally into LLC solid tumors. Quantitative CT and inductively coupled plasma optical emission spectrometry (ICP-OES) results both confirm the presence of particles in the tumor 9 days post-injection (n = 8 mice/group). A significant difference is highlighted between citrate-GNP and BSA-GNP groups (** p < 0.005, Tukey’s multiple comparisons test), confirming that the protein corona of GNPs modifies intratumoral distribution and retention of the particles. In conclusion, our investigations show that the surface passivation of GNPs influences the mechanism of cellular uptake and intratumoral distribution in vivo, highlighting the spatial heterogeneity of the solid tumor. View Full-Text
Keywords: gold nanoparticles; theranostics; in vivo computed tomography imaging; non-small cell lung cancer; in vivo biodistribution; surface passivation; inductively coupled plasma optical emission spectrometry gold nanoparticles; theranostics; in vivo computed tomography imaging; non-small cell lung cancer; in vivo biodistribution; surface passivation; inductively coupled plasma optical emission spectrometry
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MDPI and ACS Style

Terracciano, R.; Zhang, A.; Butler, E.B.; Demarchi, D.; Hafner, J.H.; Grattoni, A.; Filgueira, C.S. Effects of Surface Protein Adsorption on the Distribution and Retention of Intratumorally Administered Gold Nanoparticles. Pharmaceutics 2021, 13, 216. https://doi.org/10.3390/pharmaceutics13020216

AMA Style

Terracciano R, Zhang A, Butler EB, Demarchi D, Hafner JH, Grattoni A, Filgueira CS. Effects of Surface Protein Adsorption on the Distribution and Retention of Intratumorally Administered Gold Nanoparticles. Pharmaceutics. 2021; 13(2):216. https://doi.org/10.3390/pharmaceutics13020216

Chicago/Turabian Style

Terracciano, Rossana, Aobo Zhang, E. Brian Butler, Danilo Demarchi, Jason H. Hafner, Alessandro Grattoni, and Carly S. Filgueira. 2021. "Effects of Surface Protein Adsorption on the Distribution and Retention of Intratumorally Administered Gold Nanoparticles" Pharmaceutics 13, no. 2: 216. https://doi.org/10.3390/pharmaceutics13020216

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