Colonic Delivery of Celastrol-Loaded Layer-by-Layer Liposomes with Pectin/Trimethylated Chitosan Coating to Enhance Its Anti-Ulcerative Colitis Effects
Abstract
:1. Introduction
2. Materials and Methods
3. Results and Discussion
3.1. Synthesis and Characterization of TMC
3.2. In Vitro Mucin Mucoadhesion Assay
3.3. Characterization of Cel/PT-LbL Lipo
3.4. In Vitro Drug Release Studies in Different Simulated Gastrointestinal Media
3.5. In Vitro Intracellular Uptake Study
3.6. In Vitro Anti-Inflammatory Effects of Cel/PT-LbL Lipo
3.7. PT-LbL Lipo Preferentially Adheres to Colonic Mucosa Ex Vivo
3.8. PT-LbL Lipo Facilitates Colon Tissue Penetration
3.9. Bio-Distribution of PT-LbL Lipo after Oral Administration in Mice
3.10. In Vivo Therapeutic Effect of Cel/PT-LbL Lipo against UC
4. Conclusions
Supplementary Materials
Author Contributions
Funding
Institutional Review Board Statement
Informed Consent Statement
Date Availability Statement
Conflicts of Interest
References
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Liposomal System | Particle Size (nm) | Zeta Potential (mV) | Polydispersity Index (PDI, %) |
---|---|---|---|
Cel/Lipo | 166.68 ± 3.00 | −5.7 ± 0.15 | 20.23 ± 0.70 |
Cel/TMC Lipo | 172.57 ± 2.87 | 3.3 ± 0.32 | 20.87 ± 3.07 |
Cel/PT-LbL Lipo | 179.95 ± 4.31 | −10.0 ± 0.15 | 23.33 ± 0.59 |
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Xian, J.; Zhong, X.; Gu, H.; Wang, X.; Li, J.; Li, J.; Wu, Y.; Zhang, C.; Zhang, J. Colonic Delivery of Celastrol-Loaded Layer-by-Layer Liposomes with Pectin/Trimethylated Chitosan Coating to Enhance Its Anti-Ulcerative Colitis Effects. Pharmaceutics 2021, 13, 2005. https://doi.org/10.3390/pharmaceutics13122005
Xian J, Zhong X, Gu H, Wang X, Li J, Li J, Wu Y, Zhang C, Zhang J. Colonic Delivery of Celastrol-Loaded Layer-by-Layer Liposomes with Pectin/Trimethylated Chitosan Coating to Enhance Its Anti-Ulcerative Colitis Effects. Pharmaceutics. 2021; 13(12):2005. https://doi.org/10.3390/pharmaceutics13122005
Chicago/Turabian StyleXian, Jing, Xuemei Zhong, Huan Gu, Xiao Wang, Jiaxin Li, Jingjing Li, Yihan Wu, Chen Zhang, and Jinming Zhang. 2021. "Colonic Delivery of Celastrol-Loaded Layer-by-Layer Liposomes with Pectin/Trimethylated Chitosan Coating to Enhance Its Anti-Ulcerative Colitis Effects" Pharmaceutics 13, no. 12: 2005. https://doi.org/10.3390/pharmaceutics13122005
APA StyleXian, J., Zhong, X., Gu, H., Wang, X., Li, J., Li, J., Wu, Y., Zhang, C., & Zhang, J. (2021). Colonic Delivery of Celastrol-Loaded Layer-by-Layer Liposomes with Pectin/Trimethylated Chitosan Coating to Enhance Its Anti-Ulcerative Colitis Effects. Pharmaceutics, 13(12), 2005. https://doi.org/10.3390/pharmaceutics13122005