Next Article in Journal
Copper (II) Metallodendrimers Combined with Pro-Apoptotic siRNAs as a Promising Strategy Against Breast Cancer Cells
Next Article in Special Issue
Catechol Containing Polyelectrolyte Complex Nanoparticles as Local Drug Delivery System for Bortezomib at Bone Substitute Materials
Previous Article in Journal
Ionically Crosslinked Complex Gels Loaded with Oleic Acid-Containing Vesicles for Transdermal Drug Delivery
Previous Article in Special Issue
Targeting Activated Hepatic Stellate Cells Using Collagen-Binding Chitosan Nanoparticles for siRNA Delivery to Fibrotic Livers
Article

Assessment of Intracellular Delivery Potential of Novel Sustainable Poly(δ-decalactone)-Based Micelles

1
Pharmaceutical Sciences Laboratory, Faculty of Science and Engineering, Åbo Akademi University, 20520 Turku, Finland
2
School of Pharmacy, University of Nottingham, University Park, Nottingham NG7 2RD, UK
3
NMIMS School of Pharmacy and Technology Management, Shirpur (Maharashtra) 425405, India
*
Author to whom correspondence should be addressed.
These authors contributed equally to this work.
Pharmaceutics 2020, 12(8), 726; https://doi.org/10.3390/pharmaceutics12080726
Received: 25 June 2020 / Revised: 27 July 2020 / Accepted: 30 July 2020 / Published: 2 August 2020
(This article belongs to the Special Issue Lipid- and/or Polymer-Based Drug Delivery Systems)
Biodegradable polymers from renewable resources have attracted much attention in recent years within the biomedical field. Lately, poly(δ-decalactone) based copolymer micelles have emerged as a potential drug delivery carrier material as a sustainable alternative to fossil-based polymers. However, their intracellular drug delivery potential is not yet investigated and therefore, in this work, we report on the synthesis and cellular uptake efficiency of poly(δ-decalactone) based micelles with or without a targeting ligand. Folic acid was chosen as a model targeting ligand and Rhodamine B as a fluorescent tracer to demonstrate the straightforward functionalisation aspect of copolymers. The synthesis of block copolymers was accomplished by a combination of facile ring-opening polymerisation and click chemistry to retain the structure uniformity. The presence of folic acid on the surface of micelles with diameter ~150 nm upsurge the uptake efficiency by 1.6 fold on folate receptor overexpressing MDA-MB-231 cells indicating the attainment of targeting using ligand functionality. The drug delivery capability of these carriers was ascertained by using docetaxel as a model drug, whereby the in vitro cytotoxicity of the drug was significantly increased after incorporation in micelles 48 h post incubation. We have also investigated the possible endocytosis route of non-targeted micelles and found that caveolae-mediated endocytosis was the preferred route of uptake. This work strengthens the prospect of using novel bio-based poly(δ-decalactone) micelles as efficient multifunctional drug delivery nanocarriers towards medical applications. View Full-Text
Keywords: poly(δ-decalactone); functionalised micelles; targeted drug delivery; renewable polymer; caveolae-mediated endocytosis; intracellular delivery poly(δ-decalactone); functionalised micelles; targeted drug delivery; renewable polymer; caveolae-mediated endocytosis; intracellular delivery
Show Figures

Graphical abstract

MDPI and ACS Style

Bansal, K.K.; Özliseli, E.; Saraogi, G.K.; Rosenholm, J.M. Assessment of Intracellular Delivery Potential of Novel Sustainable Poly(δ-decalactone)-Based Micelles. Pharmaceutics 2020, 12, 726. https://doi.org/10.3390/pharmaceutics12080726

AMA Style

Bansal KK, Özliseli E, Saraogi GK, Rosenholm JM. Assessment of Intracellular Delivery Potential of Novel Sustainable Poly(δ-decalactone)-Based Micelles. Pharmaceutics. 2020; 12(8):726. https://doi.org/10.3390/pharmaceutics12080726

Chicago/Turabian Style

Bansal, Kuldeep K., Ezgi Özliseli, Gaurav K. Saraogi, and Jessica M. Rosenholm. 2020. "Assessment of Intracellular Delivery Potential of Novel Sustainable Poly(δ-decalactone)-Based Micelles" Pharmaceutics 12, no. 8: 726. https://doi.org/10.3390/pharmaceutics12080726

Find Other Styles
Note that from the first issue of 2016, MDPI journals use article numbers instead of page numbers. See further details here.

Article Access Map by Country/Region

1
Back to TopTop