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Open AccessArticle

Monitoring of Antimicrobial Drug Chloramphenicol Release from Electrospun Nano- and Microfiber Mats Using UV Imaging and Bacterial Bioreporters

1
Institute of Pharmacy, Faculty of Medicine, University of Tartu, Nooruse 1, 50411 Tartu, Estonia
2
Department of Pharmacy, University of Copenhagen, Universitetsparken 2, DK-2100 Copenhagen Ø, Denmark
3
Institute of Technology, Faculty of Natural Sciences, University of Tartu, Nooruse 1, 50411 Tartu, Estonia
4
LEO Foundation Center for Cutaneous Drug Delivery, Department of Pharmacy, University of Copenhagen, Universitetsparken 2, DK-2100 Copenhagen Ø, Denmark
*
Authors to whom correspondence should be addressed.
Pharmaceutics 2019, 11(9), 487; https://doi.org/10.3390/pharmaceutics11090487
Received: 31 August 2019 / Revised: 15 September 2019 / Accepted: 16 September 2019 / Published: 19 September 2019
(This article belongs to the Special Issue Recent Development of Electrospinning for Drug Delivery)
New strategies are continuously sought for the treatment of skin and wound infections due to increased problems with non-healing wounds. Electrospun nanofiber mats with antibacterial agents as drug delivery systems provide opportunities for the eradication of bacterial infections as well as wound healing. Antibacterial activities of such mats are directly linked with their drug release behavior. Traditional pharmacopoeial drug release testing settings are not always suitable for analyzing the release behavior of fiber mats intended for the local drug delivery. We tested and compared different drug release model systems for the previously characterized electrospun chloramphenicol (CAM)-loaded nanofiber (polycaprolactone (PCL)) and microfiber (PCL in combination with polyethylene oxide) mats with different drug release profiles. Drug release into buffer solution and hydrogel was investigated and drug concentration was determined using either high-performance liquid chromatography, ultraviolet-visible spectrophotometry, or ultraviolet (UV) imaging. The CAM release and its antibacterial effects in disc diffusion assay were assessed by bacterial bioreporters. All tested model systems enabled to study the drug release from electrospun mats. It was found that the release into buffer solution showed larger differences in the drug release rate between differently designed mats compared to the hydrogel release tests. The UV imaging method provided an insight into the interactions with an agarose hydrogel mimicking wound tissue, thus giving us information about early drug release from the mat. Bacterial bioreporters showed clear correlations between the drug release into gel and antibacterial activity of the electrospun CAM-loaded mats. View Full-Text
Keywords: antibacterial activity; bacterial bioreporters; drug release; electrospinning; microfibers; nanofibers; UV imaging antibacterial activity; bacterial bioreporters; drug release; electrospinning; microfibers; nanofibers; UV imaging
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MDPI and ACS Style

Preem, L.; Bock, F.; Hinnu, M.; Putrinš, M.; Sagor, K.; Tenson, T.; Meos, A.; Østergaard, J.; Kogermann, K. Monitoring of Antimicrobial Drug Chloramphenicol Release from Electrospun Nano- and Microfiber Mats Using UV Imaging and Bacterial Bioreporters. Pharmaceutics 2019, 11, 487.

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