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Open AccessArticle

Evaluation of Drug Delivery and Efficacy of Ciprofloxacin-Loaded Povidone Foils and Nanofiber Mats in a Wound-Infection Model Based on Ex Vivo Human Skin

1
Clinical Research Center for Hair and Skin Science, Department of Dermatology and Allergy, Charité — Universitätsmedizin Berlin, Corporate Member of Freie Universität Berlin, Humboldt-Universität zu Berlin, and Berlin Institute of Health, 10117 Berlin, Germany
2
Smart Materials, Istituto Italiano di Tecnologia, 16163 Genova, Italy
3
Advanced Light and Electron Microscopy, ZBS4, Robert Koch Institute, 13353 Berlin, Germany
*
Author to whom correspondence should be addressed.
Pharmaceutics 2019, 11(10), 527; https://doi.org/10.3390/pharmaceutics11100527
Received: 16 September 2019 / Revised: 8 October 2019 / Accepted: 10 October 2019 / Published: 12 October 2019
Topical treatment of wound infections is often a challenge due to limited drug availability at the site of infection. Topical drug delivery is an attractive option for reducing systemic side effects, provided that a more selective and sustained local drug delivery is achieved. In this study, a poorly water-soluble antibiotic, ciprofloxacin, was loaded on polyvinylpyrrolidone (PVP)-based foils and nanofiber mats using acetic acid as a solubilizer. Drug delivery kinetics, local toxicity, and antimicrobial activity were tested on an ex vivo wound model based on full-thickness human skin. Wounds of 5 mm in diameter were created on 1.5 × 1.5 cm skin blocks and treated with the investigated materials. While nanofiber mats reached the highest amount of delivered drug after 6 h, foils rapidly achieved a maximum drug concentration and maintained it over 24 h. The treatment had no effect on the overall skin metabolic activity but influenced the wound healing process, as observed using histological analysis. Both delivery systems were efficient in preventing the growth of Pseudomonas aeruginosa biofilms in ex vivo human skin. Interestingly, foils loaded with 500 µg of ciprofloxacin accomplished the complete eradication of biofilm infections with 1 × 109 bacteria/wound. We conclude that antimicrobial-loaded resorbable PVP foils and nanofiber mats are promising delivery systems for the prevention or topical treatment of infected wounds.
Keywords: wound infection; biofilm; pseudomonas aeruginosa; antimicrobial delivery; polyvinylpyrrolidone; nanofibers wound infection; biofilm; pseudomonas aeruginosa; antimicrobial delivery; polyvinylpyrrolidone; nanofibers
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MDPI and ACS Style

Rancan, F.; Contardi, M.; Jurisch, J.; Blume-Peytavi, U.; Vogt, A.; Bayer, I.S.; Schaudinn, C. Evaluation of Drug Delivery and Efficacy of Ciprofloxacin-Loaded Povidone Foils and Nanofiber Mats in a Wound-Infection Model Based on Ex Vivo Human Skin. Pharmaceutics 2019, 11, 527.

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