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Viruses 2017, 9(12), 392; https://doi.org/10.3390/v9120392

Characterization of HIV-1 Near Full-Length Proviral Genome Quasispecies from Patients with Undetectable Viral Load Undergoing First-Line HAART Therapy

1
Programa de Oncovirologia, Instituto Nacional de Câncer, Rio de Janeiro 20231-050, Brazil
2
Serviço de Doenças Infecciosas, Hospital Federal de Ipanema, Rio de Janeiro 22411-020, Brazil
3
Departamento de Genética, Universidade Federal do Rio de Janeiro, Rio de Janeiro 21944-970, Brazil
These authors contributed equally to this work.
*
Author to whom correspondence should be addressed.
Received: 1 December 2017 / Revised: 16 December 2017 / Accepted: 18 December 2017 / Published: 19 December 2017
(This article belongs to the Special Issue Homage to Mark Wainberg)
Full-Text   |   PDF [933 KB, uploaded 19 December 2017]   |  

Abstract

Increased access to highly active antiretroviral therapy (HAART) by human immunodeficiency virus postive (HIV+) individuals has become a reality worldwide. In Brazil, HAART currently reaches over half of HIV-infected subjects. In the context of a remarkable HIV-1 genetic variability, highly related variants, called quasispecies, are generated. HIV quasispecies generated during infection can influence virus persistence and pathogenicity, representing a challenge to treatment. However, the clinical relevance of minority quasispecies is still uncertain. In this study, we have determined the archived proviral sequences, viral subtype and drug resistance mutations from a cohort of HIV+ patients with undetectable viral load undergoing HAART as first-line therapy using next-generation sequencing for near full-length virus genome (NFLG) assembly. HIV-1 consensus sequences representing NFLG were obtained for eleven patients, while for another twelve varying genome coverage rates were obtained. Phylogenetic analysis showed the predominance of subtype B (83%; 19/23). Considering the minority variants, 18 patients carried archived virus harboring at least one mutation conferring antiretroviral resistance; for six patients, the mutations correlated with the current ARVs used. These data highlight the importance of monitoring HIV minority drug resistant variants and their clinical impact, to guide future regimen switches and improve HIV treatment success. View Full-Text
Keywords: HIV-1; quasispecies; minority resistance mutations; HAART; drug resistance; undetectable viral load HIV-1; quasispecies; minority resistance mutations; HAART; drug resistance; undetectable viral load
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Alves, B.M.; Siqueira, J.D.; Garrido, M.M.; Botelho, O.M.; Prellwitz, I.M.; Ribeiro, S.R.; Soares, E.A.; Soares, M.A. Characterization of HIV-1 Near Full-Length Proviral Genome Quasispecies from Patients with Undetectable Viral Load Undergoing First-Line HAART Therapy. Viruses 2017, 9, 392.

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