Genetic Diversity of Spike, 3a, 3b and E Genes of Infectious Bronchitis Viruses and Emergence of New Recombinants  in Korea

Mo, Mei-Lan; Hong, Seung-Min; Kwon, Hyuk-Joon; Kim, Il-Hwan; Song, Chang-Seon; Kim, Jae-Hong

doi:10.3390/v5020550

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Genetic Diversity of Spike, 3a, 3b and E Genes of Infectious Bronchitis Viruses and Emergence of New Recombinants in Korea

^1,3,4,†,

^1,†,

²,

^1,3,

⁵ and

^1,3,*

Laboratory of Avian Diseases, College of Veterinary Medicine, Seoul National University, Seoul 151-742, Korea

Zoonotic Disease Institute, College of Veterinary Medicine, Seoul National University, Seoul 151-742, Korea

BK21 for Veterinary Science, Seoul National University, Seoul 151-742, Korea

⁴

College of Animal Science and Technology, Guangxi University, 100 Daxue Road, Nanning, Guangxi 530005, China

⁵

College of Veterinary Medicine, Konkuk University, 1 Hwayang-dong, Gwangjin-gu, Seoul 143-701, Korea

Author to whom correspondence should be addressed.

^†

These authors contributed equally to this work.

Viruses 2013, 5(2), 550-567; https://doi.org/10.3390/v5020550

Received: 12 December 2012 / Revised: 21 January 2013 / Accepted: 24 January 2013 / Published: 31 January 2013

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Abstract

The nucleotide sequences of a region including S1, S2, 3a, 3b and E genes of twenty-seven infectious bronchitis virus (IBV) isolates in Korea between 1990–2011 were determined and phylogenetic and computational recombination analyses were conducted. The sizes of coding regions of some genes varied among IBV isolates due to deletion or insertion of nucleotides; the nucleotide similarities of S1, S2, 3a, 3b and E genes among the 27 isolates were 75.9%–100.0%, 85%–100.0%, 64.0%–100.0%, 60.4%–100.0% and 83.1%–100.0%, respectively. According to phylogenetic analysis of S1 gene, the 27 isolates were divided into five genotypes, Mass, Korean-I (K-I), QX-like, KM91-like and New cluster 1. The phylogenetic trees based on the S2, 3a, 3b, E genes and S1-S2-3a-3b-E (S1-E) region nucleotide sequences did not closely follow the clustering based on the S1 sequence. The New cluster 1 prevalent during 2009 and 2010 was not found in 2011 but QX-like viruses became prevalent in 2011. The recombination analysis revealed two new S gene recombinants, 11036 and 11052 which might have been derived from recombinations between the New cluster 1 and QX-like viruses and between the K-I and H120 (vaccine) viruses, respectively. In conclusion, multiple IBV genotypes have co-circulated; QX-like viruses have recurred and new recombinants have emerged in Korea. This has enriched molecular epidemiology information of IBV and is useful for the control of IB in Korea. View Full-Text

Keywords: infectious bronchitis virus (IBV); phylogenetic analysis; genetic diversity; recombinant; vaccine

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Mo, M.-L.; Hong, S.-M.; Kwon, H.-J.; Kim, I.-H.; Song, C.-S.; Kim, J.-H. Genetic Diversity of Spike, 3a, 3b and E Genes of Infectious Bronchitis Viruses and Emergence of New Recombinants in Korea. Viruses 2013, 5, 550-567. https://doi.org/10.3390/v5020550

AMA Style

Mo M-L, Hong S-M, Kwon H-J, Kim I-H, Song C-S, Kim J-H. Genetic Diversity of Spike, 3a, 3b and E Genes of Infectious Bronchitis Viruses and Emergence of New Recombinants in Korea. Viruses. 2013; 5(2):550-567. https://doi.org/10.3390/v5020550

Chicago/Turabian Style

Mo, Mei-Lan, Seung-Min Hong, Hyuk-Joon Kwon, Il-Hwan Kim, Chang-Seon Song, and Jae-Hong Kim. 2013. "Genetic Diversity of Spike, 3a, 3b and E Genes of Infectious Bronchitis Viruses and Emergence of New Recombinants in Korea" Viruses 5, no. 2: 550-567. https://doi.org/10.3390/v5020550

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Genetic Diversity of Spike, 3a, 3b and E Genes of Infectious Bronchitis Viruses and Emergence of New Recombinants in Korea

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