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Article

Detection of Feline Coronavirus Variants in Cats without Feline Infectious Peritonitis

1
Clinic of Small Animal Medicine, Centre for Clinical Veterinary Medicine, LMU Munich, Veterinärstraße 13, 80539 Munich, Germany
2
Section of Clinical and Comparative Neuropathology, Institute of Veterinary Pathology, Centre for Clinical Veterinary Medicine, LMU Munich, Veterinärstraße 13, 80539 Munich, Germany
3
Clinical Laboratory, Department of Clinical Diagnostics and Services, Center for Clinical Studies, Vetsuisse Faculty, University of Zurich, Winterthurerstrasse 260, 8057 Zurich, Switzerland
*
Author to whom correspondence should be addressed.
Academic Editors: Julia A. Beatty and Séverine Tasker
Viruses 2022, 14(8), 1671; https://doi.org/10.3390/v14081671
Received: 30 April 2022 / Revised: 25 July 2022 / Accepted: 27 July 2022 / Published: 29 July 2022
(This article belongs to the Special Issue Feline Viruses and Viral Diseases 2.0)
(1) Background: This study aimed to detect feline coronavirus (FCoV) and characterize spike (S) gene mutation profiles in cats suffering from diseases other than feline infectious peritonitis (FIP) using commercial real-time reverse transcription polymerase chain reaction (RT-qPCR) and reevaluating results by sequencing. (2) Methods: In 87 cats in which FIP was excluded by histopathology and immunohistochemistry, FCoV 7b gene and S gene mutation RT-qPCR was performed prospectively on incisional biopsies and fine-needle aspirates of different organs, body fluids, and feces. Samples positive for S gene mutations or mixed FCoV underwent sequencing. (3) Results: In 21/87 cats, FCoV RNA was detectable. S gene mutations were detected by commercial RT-qPCR (and a diagnostic algorithm that was used at the time of sample submission) in at least one sample in 14/21 cats (66.7%), with only mutated FCoV in 2/21, only mixed in 1/21, and different results in 11/21 cats; in the remaining 7/21 cats, RNA load was too low to differentiate. However, sequencing of 8 tissue samples and 8 fecal samples of 9 cats did not confirm mutated FCoV in any of the FCoV RNA-positive cats without FIP. (4) Conclusions: Sequencing results did not confirm results of the commercial S gene mutation RT-qPCR. View Full-Text
Keywords: FCoV; FIP; RT-PCR; S gene mutation; sequencing; IHC; immunohistochemistry FCoV; FIP; RT-PCR; S gene mutation; sequencing; IHC; immunohistochemistry
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MDPI and ACS Style

Jähne, S.; Felten, S.; Bergmann, M.; Erber, K.; Matiasek, K.; Meli, M.L.; Hofmann-Lehmann, R.; Hartmann, K. Detection of Feline Coronavirus Variants in Cats without Feline Infectious Peritonitis. Viruses 2022, 14, 1671. https://doi.org/10.3390/v14081671

AMA Style

Jähne S, Felten S, Bergmann M, Erber K, Matiasek K, Meli ML, Hofmann-Lehmann R, Hartmann K. Detection of Feline Coronavirus Variants in Cats without Feline Infectious Peritonitis. Viruses. 2022; 14(8):1671. https://doi.org/10.3390/v14081671

Chicago/Turabian Style

Jähne, Stéphanie, Sandra Felten, Michèle Bergmann, Katharina Erber, Kaspar Matiasek, Marina L. Meli, Regina Hofmann-Lehmann, and Katrin Hartmann. 2022. "Detection of Feline Coronavirus Variants in Cats without Feline Infectious Peritonitis" Viruses 14, no. 8: 1671. https://doi.org/10.3390/v14081671

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