Next Article in Journal
Mitigating Future Respiratory Virus Pandemics: New Threats and Approaches to Consider
Previous Article in Journal
Successful Direct Acting Antiviral Therapy in Chronic Hepatitis C Normalizes IFNγ and IL2 Production in T Cells Together with TLR8 Expression and Functionality in Peripheral Blood Mononuclear Cells
Open AccessCommunication

First Report on Natural Infection of Nodavirus in an Echinodermata, Sea Cucumber (Apostichopus japonicas)

1
School of Marine Sciences, Ningbo University, Ningbo 315311, China
2
Yellow Sea Fisheries Research Institute, Chinese Academy of Fishery Sciences, Function Laboratory for Marine Fisheries Science and Food Production Processes (Qingdao), National Laboratory for Marine Science and Technology, Key Laboratory of Maricultural Organism Disease Control, Ministry of Agriculture, Qingdao Key Laboratory of Mariculture Epidemiology and Biosecurity, Qingdao 266071, China
*
Authors to whom correspondence should be addressed.
Academic Editor: Karla Helbig
Viruses 2021, 13(4), 636; https://doi.org/10.3390/v13040636
Received: 4 February 2021 / Revised: 23 February 2021 / Accepted: 24 February 2021 / Published: 8 April 2021
(This article belongs to the Section Animal Viruses)
Cross-species transmission of emerging viruses happens occasionally due to epidemiological, biological, and ecological factors, and it has caused more concern recently. Covert mortality nodavirus (CMNV) was revealed to be a unique shrimp virus that could cross species barrier to infect vertebrate fish. In the present study, CMNV reverse transcription-nested PCR (RT-nPCR)-positive samples were identified from farmed sea cucumber (Apostichopus japonicas) in the CMNV host range investigation. The amplicons of RT-nPCR from sea cucumber were sequenced, and its sequences showed 100% identity with the RNA-dependent RNA polymerase gene of the original CMNV isolate. Histopathological analysis revealed pathologic changes, including karyopyknosis and vacuolation of the epithelial cells, in the sea cucumber intestinal tissue. The extensive positive hybridization signals with CMNV probe were shown in the damaged epithelial cells in the in situ hybridization assay. Meanwhile, transmission electron microscopy analysis revealed CMNV-like virus particles in the intestine epithelium. All the results indicated that the sea cucumber, an Echinodermata, is a new host of CMNV. This study supplied further evidence of the wide host range of CMNV and also reminded us to pay close attention to its potential risk to threaten different aquaculture animal species. View Full-Text
Keywords: covert mortality nodavirus (CMNV); natural infection; sea cucumber (Apostichopus japonicas); in situ hybridization; TEM covert mortality nodavirus (CMNV); natural infection; sea cucumber (Apostichopus japonicas); in situ hybridization; TEM
Show Figures

Figure 1

MDPI and ACS Style

Wang, C.; Yao, L.; Wang, W.; Sang, S.; Hao, J.; Li, C.; Zhang, Q. First Report on Natural Infection of Nodavirus in an Echinodermata, Sea Cucumber (Apostichopus japonicas). Viruses 2021, 13, 636. https://doi.org/10.3390/v13040636

AMA Style

Wang C, Yao L, Wang W, Sang S, Hao J, Li C, Zhang Q. First Report on Natural Infection of Nodavirus in an Echinodermata, Sea Cucumber (Apostichopus japonicas). Viruses. 2021; 13(4):636. https://doi.org/10.3390/v13040636

Chicago/Turabian Style

Wang, Chong; Yao, Liang; Wang, Wei; Sang, Songwen; Hao, Jingwei; Li, Chenghua; Zhang, Qingli. 2021. "First Report on Natural Infection of Nodavirus in an Echinodermata, Sea Cucumber (Apostichopus japonicas)" Viruses 13, no. 4: 636. https://doi.org/10.3390/v13040636

Find Other Styles
Note that from the first issue of 2016, MDPI journals use article numbers instead of page numbers. See further details here.

Article Access Map by Country/Region

1
Search more from Scilit
 
Search
Back to TopTop