Seoul Virus in Pet and Feeder Rats in The Netherlands
Abstract
:1. Introduction
2. Materials and Methods
2.1. Study Design
2.2. Dissection
2.3. Molecular Diagnostics
2.4. Serology
2.5. Comparison of RT-qPCR Detection in Fresh and Frozen Tissue
2.6. Ethics Statement
3. Results
3.1. Rats
3.2. SEOV Infections
3.3. Questionnaires
3.4. Phylogenetics
3.5. Comparison of RT-qPCR Detection in Fresh and Frozen Material
4. Discussion
Author Contributions
Funding
Institutional Review Board Statement
Informed Consent Statement
Data Availability Statement
Acknowledgments
Conflicts of Interest
References
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Source | Number of Participants | Total Number of Rats Collected | Number of Rats per Participant (Range) |
---|---|---|---|
Private owners via veterinarians | 8 veterinarians 1 | 29 | 2–11 |
Ratteries and rat shelters | 5 ratteries, 2 shelters | 56 | 5–10 |
Commercial breeders and traders | 9 | 90 | 10 |
Source | Total Number of Rats | Nr. of Rats RT-qPCR+ (Percentage) | Nr. of Rats ELISA+ Based on Cut-Off 1 (Percentage) | Nr. of Rats ELISA+ Based on Mixture Model (Percentage) |
---|---|---|---|---|
Private owners via veterinarians | 29 | 1 (3.4%) | 1 (3.4%) | 1 (3.4%) |
Ratteries and rat shelters | 56 | 2 (3.6%) | 3 (5.4%) | 1 (1.8%) |
Commercial breeders and traders | 90 | 11 (12.2%) | 11 (12.2%) | 9 (10.0%) |
Rat ID | RT-qPCR Result on Fresh Tissue (Cq Value) | RT-qPCR Result on Frozen Tissue (Cq Value) |
---|---|---|
RN2352 | - | - |
RN2353 | +(27.83) | - |
RN2354 | +(30.11) | +(26.53) |
RN2355 | - | - |
RN2356 | - | +(28.38) |
RN2357 | +(25.61) | +(25.72) |
RN2358 | +(23.68) | +(22.61) |
RN2359 | +(26.67) | +(27.91) |
RN2360 | - | - |
RN2361 | +(29.84) | - |
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Cuperus, T.; de Vries, A.; Hoornweg, T.E.; Fonville, M.; Jaarsma, R.I.; Opsteegh, M.; Maas, M. Seoul Virus in Pet and Feeder Rats in The Netherlands. Viruses 2021, 13, 443. https://doi.org/10.3390/v13030443
Cuperus T, de Vries A, Hoornweg TE, Fonville M, Jaarsma RI, Opsteegh M, Maas M. Seoul Virus in Pet and Feeder Rats in The Netherlands. Viruses. 2021; 13(3):443. https://doi.org/10.3390/v13030443
Chicago/Turabian StyleCuperus, Tryntsje, Ankje de Vries, Tabitha E. Hoornweg, Manoj Fonville, Ryanne I. Jaarsma, Marieke Opsteegh, and Miriam Maas. 2021. "Seoul Virus in Pet and Feeder Rats in The Netherlands" Viruses 13, no. 3: 443. https://doi.org/10.3390/v13030443