The Picornavirus Precursor 3CD Has Different Conformational Dynamics Compared to 3Cpro and 3Dpol in Functionally Relevant Regions
Abstract
:1. Introduction
2. Materials and Methods
2.1. Protein Expression and Isotopic Labeling
2.2. Protein Purification
2.3. NMR Sample Preparation
2.4. δ1-13. CH3 Resonance Assignments for 3C, 3D, and 3CD
2.5. Determination of Methyl Axis Order Parameters
2.6. CPMG Relaxation Dispersion
2.7. H and 13C CEST
3. Results
3.1. Ile Methyl Resonance Assignments for 3C
3.2. Conformational Dynamics of 3C on the Micro-to-Millisecond Timescale
3.3. Conformational Dynamics of 3C on the Millisecond-Second Timescale
3.4. Conformational Dynamics of 3C on the Picosecond-Nanosecond Timescale
3.5. Conformational Dynamics of 3CD: 3C Domain
3.6. Ile Methyl Resonance Assignments for 3D
3.7. Conformational Dynamics of 3D on the Microsecond to Second Timescale
3.8. Conformational Dynamics of 3D on the Picosecond to Nanosecond Timescale
3.9. Conformational Dynamics of 3CD: 3D Domain
3.10. Domain Independence in 3CD
4. Discussion
4.1. Comparison of 3C and 3CD Conformational Dynamics
4.2. Comparison of 3D and 3CD Conformational Dynamics
5. Conclusions
Supplementary Materials
Author Contributions
Funding
Institutional Review Board Statement
Informed Consent Statement
Data Availability Statement
Acknowledgments
Conflicts of Interest
References
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Winston, D.S.; Boehr, D.D. The Picornavirus Precursor 3CD Has Different Conformational Dynamics Compared to 3Cpro and 3Dpol in Functionally Relevant Regions. Viruses 2021, 13, 442. https://doi.org/10.3390/v13030442
Winston DS, Boehr DD. The Picornavirus Precursor 3CD Has Different Conformational Dynamics Compared to 3Cpro and 3Dpol in Functionally Relevant Regions. Viruses. 2021; 13(3):442. https://doi.org/10.3390/v13030442
Chicago/Turabian StyleWinston, Dennis S., and David D. Boehr. 2021. "The Picornavirus Precursor 3CD Has Different Conformational Dynamics Compared to 3Cpro and 3Dpol in Functionally Relevant Regions" Viruses 13, no. 3: 442. https://doi.org/10.3390/v13030442