Next Article in Journal
Application of Advanced Light Microscopy to the Study of HIV and Its Interactions with the Host
Next Article in Special Issue
LAMP-Coupled CRISPR–Cas12a Module for Rapid and Sensitive Detection of Plant DNA Viruses
Previous Article in Journal
In Vitro Evaluation of Anti-Rift Valley Fever Virus, Antioxidant and Anti-Inflammatory Activity of South African Medicinal Plant Extracts
Previous Article in Special Issue
The AC2 Protein of a Bipartite Geminivirus Stimulates the Transcription of the BV1 Gene through Abscisic Acid Responsive Promoter Elements
Article

The 28 Ser Amino Acid of Cucumber Mosaic Virus Movement Protein Has a Role in Symptom Formation and Plasmodesmata Localization

1
Centre for Agricultural Research, Plant Protection Institute, Herman Ottó Street 15., H-1022 Budapest, Hungary
2
Department of Plant Pathology, Faculty of Horticultural Science, Szent István University, Villányi Street 29-43., H-1118 Budapest, Hungary
3
Centre for Agricultural Research, Agricultural Institute, Brunszvik Street 2, H-2462 Martonvásár, Hungary
*
Author to whom correspondence should be addressed.
Academic Editors: Eugene Savenkov and Katalin Nemes
Viruses 2021, 13(2), 222; https://doi.org/10.3390/v13020222
Received: 22 December 2020 / Revised: 26 January 2021 / Accepted: 28 January 2021 / Published: 31 January 2021
(This article belongs to the Special Issue Molecular Plant-Virus Interactions)
Cucumber mosaic virus (CMV, Cucumovirus, Bromoviridae) is an economically significant virus infecting important horticultural and field crops. Current knowledge regarding the specific functions of its movement protein (MP) is still incomplete. In the present study, potential post-translational modification sites of its MP were assayed with mutant viruses: MP/S28A, MP/S28D, MP/S120A and MP/S120D. Ser28 was identified as an important factor in viral pathogenicity on Nicotiana tabacum cv. Xanthi, Cucumis sativus and Chenopodium murale. The subcellular localization of GFP-tagged movement proteins was determined with confocal laser-scanning microscopy. The wild type movement protein fused to green fluorescent protein (GFP) (MP-eGFP) greatly colocalized with callose at plasmodesmata, while MP/S28A-eGFP and MP/S28D-eGFP were detected as punctate spots along the cell membrane without callose colocalization. These results underline the importance of phosphorylatable amino acids in symptom formation and provide data regarding the essential factors for plasmodesmata localization of CMV MP. View Full-Text
Keywords: cucumber mosaic virus; movement protein; plasmodesmata localization signal; cell-to-cell movement; host plant-virus interaction cucumber mosaic virus; movement protein; plasmodesmata localization signal; cell-to-cell movement; host plant-virus interaction
Show Figures

Figure 1

MDPI and ACS Style

Sáray, R.; Fábián, A.; Palkovics, L.; Salánki, K. The 28 Ser Amino Acid of Cucumber Mosaic Virus Movement Protein Has a Role in Symptom Formation and Plasmodesmata Localization. Viruses 2021, 13, 222. https://doi.org/10.3390/v13020222

AMA Style

Sáray R, Fábián A, Palkovics L, Salánki K. The 28 Ser Amino Acid of Cucumber Mosaic Virus Movement Protein Has a Role in Symptom Formation and Plasmodesmata Localization. Viruses. 2021; 13(2):222. https://doi.org/10.3390/v13020222

Chicago/Turabian Style

Sáray, Réka, Attila Fábián, László Palkovics, and Katalin Salánki. 2021. "The 28 Ser Amino Acid of Cucumber Mosaic Virus Movement Protein Has a Role in Symptom Formation and Plasmodesmata Localization" Viruses 13, no. 2: 222. https://doi.org/10.3390/v13020222

Find Other Styles
Note that from the first issue of 2016, MDPI journals use article numbers instead of page numbers. See further details here.

Article Access Map by Country/Region

1
Back to TopTop