Next Article in Journal
Human Papillomaviruses-Associated Cancers: An Update of Current Knowledge
Next Article in Special Issue
High-Throughput Sequencing of Small RNAs for Diagnostics of Grapevine Viruses and Viroids in Russia
Previous Article in Journal
Geographic Partitioning of Dengue Virus Transmission Risk in Florida
Previous Article in Special Issue
Metagenomic Studies of Viruses in Weeds and Wild Plants: A Powerful Approach to Characterise Variable Virus Communities
Article

Characterization of Spanish Olive Virome by High Throughput Sequencing Opens New Insights and Uncertainties

1
Centro de Protección Vegetal y Biotecnología, Instituto Valenciano de Investigaciones Agrarias (IVIA), Ctra, Moncada-Náquera Km 4.5, 46113 Moncada, Spain
2
Laboratorio de Producción y Sanidad Vegetal de Jaén, Junta de Andalucía, Sierra Morena, 12b, 23620 Mengíbar, Spain
*
Author to whom correspondence should be addressed.
Academic Editor: Nuredin Habili
Viruses 2021, 13(11), 2233; https://doi.org/10.3390/v13112233
Received: 12 October 2021 / Revised: 1 November 2021 / Accepted: 2 November 2021 / Published: 6 November 2021
The use of high throughput sequencing (HTS) for the analysis of Spanish olive trees showing leaf yellowing discoloration, defoliation, and/or decline has provided new insights into the olive viruses present in Spain and has opened discussions about the pros and cons of these technologies for diagnostic purposes. In this study, we report for the first time in Spanish orchards the presence of olive leaf yellowing-associated virus (OLYaV), for which the second full coding sequence has been determined. This virus has also been detected in a putative vector, the psyllid Euphyllura olivina. In addition, the presence in Spain of Olea europaea geminivirus (OEGV), recently reported in Italy, has been confirmed, and the full-length sequence of two isolates was obtained by HTS and Sanger sequencing. These results, as well as the detection of other viral sequences related to olive latent virus 3 (OLV-3) and olive viral satellite RNA, raises questions on the biological significance of the findings, about the requirement of standardization on the interpretation of HTS results, and the necessity of additional tests to confirm the relevance of the HTS detection of viral sequences. View Full-Text
Keywords: olive; OLYaV; OEGV; OLV-3; HTS olive; OLYaV; OEGV; OLV-3; HTS
Show Figures

Figure 1

MDPI and ACS Style

Ruiz-García, A.B.; Canales, C.; Morán, F.; Ruiz-Torres, M.; Herrera-Mármol, M.; Olmos, A. Characterization of Spanish Olive Virome by High Throughput Sequencing Opens New Insights and Uncertainties. Viruses 2021, 13, 2233. https://doi.org/10.3390/v13112233

AMA Style

Ruiz-García AB, Canales C, Morán F, Ruiz-Torres M, Herrera-Mármol M, Olmos A. Characterization of Spanish Olive Virome by High Throughput Sequencing Opens New Insights and Uncertainties. Viruses. 2021; 13(11):2233. https://doi.org/10.3390/v13112233

Chicago/Turabian Style

Ruiz-García, Ana B., Celia Canales, Félix Morán, Manuel Ruiz-Torres, Magdalena Herrera-Mármol, and Antonio Olmos. 2021. "Characterization of Spanish Olive Virome by High Throughput Sequencing Opens New Insights and Uncertainties" Viruses 13, no. 11: 2233. https://doi.org/10.3390/v13112233

Find Other Styles
Note that from the first issue of 2016, MDPI journals use article numbers instead of page numbers. See further details here.

Article Access Map by Country/Region

1
Back to TopTop