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Article

Identification and Prevalence of Phascolarctid Gammaherpesvirus Types 1 and 2 in South Australian Koala Populations

School of Animal and Veterinary Sciences, The University of Adelaide, Roseworthy campus, 5371 Adelaide, Australia
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Viruses 2020, 12(9), 948; https://doi.org/10.3390/v12090948
Received: 20 July 2020 / Revised: 20 August 2020 / Accepted: 22 August 2020 / Published: 27 August 2020
(This article belongs to the Section Animal Viruses)
To determine Phascolarctid gammaherpesviruses (PhaHV) infection in South Australian koala populations, 80 oropharyngeal swabs from wild-caught and 87 oropharyngeal spleen samples and swabs from euthanased koalas were tested using two specific PCR assays developed to detect PhaHV-1 and PhaHV-2. In wild-caught koalas, active shedding of PhaHV was determined by positive oropharyngeal samples in 72.5% (58/80) of animals, of which 44.8% (26/58) had PhaHV-1, 20.7% (12/58) PhaHV-2 and 34.5% (20/58) both viral subtypes. In the euthanased koalas, systemic infection was determined by positive PCR in spleen samples and found in 72.4% (63/87) of koalas. Active shedding was determined by positive oropharyngeal results and found in 54.0% (47/87) of koalas. Koalas infected and actively shedding PhaHV-1 alone, PhaHV-2 alone or shedding both viral subtypes were 48.9% (23/47), 14.9% (7/47) and 36.2% (17/47), respectively. Only 45.9% (40/87) were not actively shedding, of which 40.0% (16/40) of these had systemic infections. Both wild-caught and euthanased koalas actively shedding PhaHV-2 were significantly more likely to be actively shedding both viral subtypes. Active shedding of PhaHV-2 had a significant negative correlation with BCS in the euthanased cohort, and active shedding of PhaHV-1 had a significant positive relationship with age in both wild-caught and euthanased cohorts. View Full-Text
Keywords: Phascolarctid gammaherpesvirus; koala; South Australia; PhaHV-1; PhaHV-2 Phascolarctid gammaherpesvirus; koala; South Australia; PhaHV-1; PhaHV-2
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MDPI and ACS Style

Kasimov, V.; Stephenson, T.; Speight, N.; Chaber, A.-L.; Boardman, W.; Easther, R.; Hemmatzadeh, F. Identification and Prevalence of Phascolarctid Gammaherpesvirus Types 1 and 2 in South Australian Koala Populations. Viruses 2020, 12, 948. https://doi.org/10.3390/v12090948

AMA Style

Kasimov V, Stephenson T, Speight N, Chaber A-L, Boardman W, Easther R, Hemmatzadeh F. Identification and Prevalence of Phascolarctid Gammaherpesvirus Types 1 and 2 in South Australian Koala Populations. Viruses. 2020; 12(9):948. https://doi.org/10.3390/v12090948

Chicago/Turabian Style

Kasimov, Vasilli, Tamsyn Stephenson, Natasha Speight, Anne-Lise Chaber, Wayne Boardman, Ruby Easther, and Farhid Hemmatzadeh. 2020. "Identification and Prevalence of Phascolarctid Gammaherpesvirus Types 1 and 2 in South Australian Koala Populations" Viruses 12, no. 9: 948. https://doi.org/10.3390/v12090948

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