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Open AccessArticle

Characterization of Port Bolivar Virus, a Novel Entomobirnavirus (Birnaviridae) Isolated from Mosquitoes Collected in East Texas, USA

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Department of Pathology, The University of Texas Medical Branch, Galveston, TX 77555-0609, USA
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Center for Biodefense and Emerging Infectious Diseases, University of Texas Medical Branch, 301 University Blvd., Galveston, TX 77555-0609, USA
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Center for Tropical Diseases, University of Texas Medical Branch, 301 University Blvd., Galveston, TX 77555-0609, USA
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Institute for Human Infection and Immunity, University of Texas Medical Branch, 301 University Blvd., Galveston, TX 77555-0610, USA
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World Reference Center for Emerging Viruses and Arboviruses, University of Texas Medical Branch, 301 University Blvd., Galveston, TX 77555-0609, USA
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Galveston County Mosquito Control, 5115 Highway 3, Dickinson, TX 77539, USA
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Department of Biochemistry and Molecular Biology, University of Texas Medical Branch, 301 University Blvd., Galveston, TX 77555, USA
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School of Chemistry and Molecular Biosciences, The University of Queensland, St Lucia, QLD 4072, Australia
*
Author to whom correspondence should be addressed.
Current affiliation: Arbovirus-Entomology Laboratory, Texas Department of State Health Services, 1100 West 49th Street, Austin, TX 78756, USA.
Viruses 2020, 12(4), 390; https://doi.org/10.3390/v12040390
Received: 13 March 2020 / Revised: 24 March 2020 / Accepted: 26 March 2020 / Published: 31 March 2020
This report describes and characterizes a novel entomobirnavirus, designated Port Bolivar virus (PTBV), that was isolated from a pool of Aedes sollicitans mosquitoes collected in a saltwater marsh in East Texas, USA. Full genome sequencing and phylogenetic analyses indicate that PTBV is distinct but genetically related to Drosophila X virus and mosquito X virus, which are assigned to species in the genus Entomobirnavirus, family Birnaviridae. PTBV produced cytopathic effect (CPE) in cultures of mosquito (C6/36) cells, but not in Vero cell cultures. Ultrastructural studies of PTBV in infected C6/36 cells demonstrated unenveloped virus particles about 55 nm in diameter. View Full-Text
Keywords: Birnaviridae; Entomobirnavirus; Port Bolivar virus; mosquito viruses Birnaviridae; Entomobirnavirus; Port Bolivar virus; mosquito viruses
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MDPI and ACS Style

Tesh, R.B.; Bolling, B.G.; Guzman, H.; Popov, V.L.; Wilson, A.; Widen, S.G.; Wood, T.G.; Walker, P.J.; Vasilakis, N. Characterization of Port Bolivar Virus, a Novel Entomobirnavirus (Birnaviridae) Isolated from Mosquitoes Collected in East Texas, USA. Viruses 2020, 12, 390.

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