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Open AccessArticle

The 40 kDa Linear Polyethylenimine Inhibits Porcine Reproductive and Respiratory Syndrome Virus Infection by Blocking Its Attachment to Permissive Cells

by 1,2,†, 1,2,†, 1,2, 1,2, 1,2, 1,2, 1,2, 3 and 1,2,*
1
Department of Preventive Veterinary Medicine, College of Veterinary Medicine, Northwest A&F University, Yangling 712100, China
2
Scientific Observing and Experimental Station of Veterinary Pharmacology and Veterinary Biotechnology, Ministry of Agriculture, Yangling 712100, China
3
Molecular Virology Laboratory, VA-MD College of Veterinary Medicine, University of Maryland, College Park, MD 20742, USA
*
Author to whom correspondence should be addressed.
These authors contributed equally to this work.
Viruses 2019, 11(9), 876; https://doi.org/10.3390/v11090876
Received: 26 July 2019 / Revised: 9 September 2019 / Accepted: 17 September 2019 / Published: 19 September 2019
(This article belongs to the Special Issue Antiviral Agents)
Porcine reproductive and respiratory syndrome (PRRS) is one of the most economically devastating infectious diseases in pigs worldwide. The causative agent is the PRRS virus (PRRSV). In this study, we explored polyethylenimine (PEI), a cationic polymer with different forms (linear or branched), to inhibit the replication of PRRSV. Our results demonstrate that the linear but not the 40 kDa branched PEI, or the 25 kDa linear PEI, were well tolerated in cultured cells and exhibited a broad-spectrum inhibition of heterogeneous PRRSV-2 isolates in both MARC-145 cells and primary porcine pulmonary alveolar macrophages (PAMs). Further analysis suggests that PEI could prevent the attachment of PRRSV virions to the susceptible cells. Notably, PEI had a minimal effect on PRRSV internalization in MARC-145 cells, whereas PEI promoted the internalization of PRRSV virions in PAMs, which suggests that these two types of cells might have different internalization processes of PRRSV virions. In conclusion, our data demonstrate that PEI could be used as a novel inhibitor against PRRSV. View Full-Text
Keywords: PRRSV; polyethylenimine; PEI; virion internalization; antiviral; endocytosis PRRSV; polyethylenimine; PEI; virion internalization; antiviral; endocytosis
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MDPI and ACS Style

Wang, J.; Li, J.; Wang, N.; Ji, Q.; Li, M.; Nan, Y.; Zhou, E.-M.; Zhang, Y.; Wu, C. The 40 kDa Linear Polyethylenimine Inhibits Porcine Reproductive and Respiratory Syndrome Virus Infection by Blocking Its Attachment to Permissive Cells. Viruses 2019, 11, 876. https://doi.org/10.3390/v11090876

AMA Style

Wang J, Li J, Wang N, Ji Q, Li M, Nan Y, Zhou E-M, Zhang Y, Wu C. The 40 kDa Linear Polyethylenimine Inhibits Porcine Reproductive and Respiratory Syndrome Virus Infection by Blocking Its Attachment to Permissive Cells. Viruses. 2019; 11(9):876. https://doi.org/10.3390/v11090876

Chicago/Turabian Style

Wang, Jie; Li, Jie; Wang, Nana; Ji, Qi; Li, Mingshuo; Nan, Yuchen; Zhou, En-Min; Zhang, Yanjin; Wu, Chunyan. 2019. "The 40 kDa Linear Polyethylenimine Inhibits Porcine Reproductive and Respiratory Syndrome Virus Infection by Blocking Its Attachment to Permissive Cells" Viruses 11, no. 9: 876. https://doi.org/10.3390/v11090876

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