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Assessing the Potential Interactions between Cellular miRNA and Arboviral Genomic RNA in the Yellow Fever Mosquito, Aedes aegypti

1
Unit of Arboviruses and Insect Vectors, Department of Virology, Institut Pasteur, 75015 Paris, France
2
National Health Research Institutes, National Institute of Infectious Diseases and Vaccinology, Miaoli 35053, Taiwan
3
National Mosquito-Borne Diseases Control Research Center, Miaoli 35053, Taiwan
4
MRC-University of Glasgow Centre for Virus Research, Glasgow G61 1QH, Scotland, UK
*
Authors to whom correspondence should be addressed.
Viruses 2019, 11(6), 540; https://doi.org/10.3390/v11060540
Received: 2 May 2019 / Revised: 4 June 2019 / Accepted: 5 June 2019 / Published: 10 June 2019
(This article belongs to the Special Issue Transmission Dynamics of Insect Viruses)
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Abstract

Although the role of exogenous small interfering RNA (siRNA) and P-element induced wimpy testis (PIWI)-interacting RNA (piRNA) pathways in mosquito antiviral immunity is increasingly better understood, there is still little knowledge regarding the role of mosquito cellular microRNA (miRNA). Identifying direct interactions between the mosquito miRNAs and the RNA genome of arboviruses and choosing the relevant miRNA candidates to explore resulting antiviral mechanisms are critical. Here, we carried out genomic analyses to identify Aedes aegypti miRNAs that potentially interact with various lineages and genotypes of chikungunya, dengue, and Zika viruses. By using prediction tools with distinct algorithms, several miRNA binding sites were commonly found within different genotypes/and or lineages of each arbovirus. We further analyzed those miRNAs that could target more than one arbovirus, required a low energy threshold to form miRNA-viralRNA (vRNA) complexes, and predicted potential RNA structures using RNAhybrid software. We predicted miRNA candidates that might participate in regulating arboviral replication in Ae. aegypti. Even without any experimental validation, which should be done as a next step, this study can shed further light on the role of miRNA in mosquito innate immunity and targets for future studies. View Full-Text
Keywords: Aedes aegypti; arboviruses; chikungunya; dengue; Zika; miRNA Aedes aegypti; arboviruses; chikungunya; dengue; Zika; miRNA
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Yen, P.-S.; Chen, C.-H.; Sreenu, V.; Kohl, A.; Failloux, A.-B. Assessing the Potential Interactions between Cellular miRNA and Arboviral Genomic RNA in the Yellow Fever Mosquito, Aedes aegypti. Viruses 2019, 11, 540.

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