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Viruses 2019, 11(4), 351; https://doi.org/10.3390/v11040351 (registering DOI)

Mitovirus and Mitochondrial Coding Sequences from Basal Fungus Entomophthora muscae

1
Department of Microbiology and Program in Virology, Harvard Medical School, Boston, MA 02115, USA
2
Instituto de Patología Vegetal, Centro de Investigaciones Agropecuarias, Instituto Nacional de Tecnología Agropecuaria (IPAVE-CIAP-INTA), Córdoba X5020ICA, Argentina
3
U.S. Department of Energy Joint Genome Institute, Walnut Creek, CA 94598, USA
4
Department of Plant and Microbial Biology, University of California Berkeley, Berkeley, CA 94720, USA
5
Department of Plant and Environmental Sciences, University of Copenhagen, DK-1871 Frederiksberg, Denmark
*
Author to whom correspondence should be addressed.
Received: 29 March 2019 / Revised: 11 April 2019 / Accepted: 15 April 2019 / Published: 17 April 2019
(This article belongs to the Special Issue Mycoviruses)
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Abstract

Fungi constituting the Entomophthora muscae species complex (members of subphylum Entomophthoromycotina, phylum Zoopagamycota) commonly kill their insect hosts and manipulate host behaviors in the process. In this study, we made use of public transcriptome data to identify and characterize eight new species of mitoviruses associated with several different E. muscae isolates. Mitoviruses are simple RNA viruses that replicate in host mitochondria and are frequently found in more phylogenetically apical fungi (members of subphylum Glomeromyoctina, phylum Mucoromycota, phylum Basidiomycota and phylum Ascomycota) as well as in plants. E. muscae is the first fungus from phylum Zoopagomycota, and thereby the most phylogenetically basal fungus, found to harbor mitoviruses to date. Multiple UGA (Trp) codons are found not only in each of the new mitovirus sequences from E. muscae but also in mitochondrial core-gene coding sequences newly assembled from E. muscae transcriptome data, suggesting that UGA (Trp) is not a rarely used codon in the mitochondria of this fungus. The presence of mitoviruses in these basal fungi has possible implications for the evolution of these viruses. View Full-Text
Keywords: database mining; Entomophthora; Entomophthoromycotina; fungal virus; mitochondrion; mycovirus; virus discovery; Mitovirus; Narnaviridae database mining; Entomophthora; Entomophthoromycotina; fungal virus; mitochondrion; mycovirus; virus discovery; Mitovirus; Narnaviridae
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Nibert, M.L.; Debat, H.J.; Manny, A.R.; Grigoriev, I.V.; De Fine Licht, H.H. Mitovirus and Mitochondrial Coding Sequences from Basal Fungus Entomophthora muscae. Viruses 2019, 11, 351.

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