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Viruses 2019, 11(4), 350;

Whole-Genome Analysis of Human Papillomavirus Type 16 Prevalent in Japanese Women with or without Cervical Lesions

Department of Obstetrics and Gynecology, Showa University School of Medicine, Shinagawa-ku, Tokyo 142-8666, Japan
Pathogen Genomics Center, National Institute of Infectious Diseases, Musashi-murayama, Tokyo 208-0011, Japan
Department of Obstetrics and Gynecology, Faculty of Medicine, University of Tsukuba, Tsukuba, Ibaraki 305-8575, Japan
Department of Obstetrics and Gynecology, Keio University School of Medicine, Shinjuku-ku, Tokyo 160-0016, Japan
Division of Carcinogenesis and Cancer Prevention, National Cancer Center Research Institute, Chuo-ku, Tokyo 104-0045, Japan
Author to whom correspondence should be addressed.
Received: 25 February 2019 / Revised: 12 April 2019 / Accepted: 13 April 2019 / Published: 16 April 2019
(This article belongs to the Section Animal Viruses)
PDF [1457 KB, uploaded 16 April 2019]
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Recent large-scale genomics studies of human papillomaviruses (HPVs) have shown a high level of genomic variability of HPV16, the most prevalent genotype in HPV-associated malignancies, and provided new insights into the biological and clinical relevance of its genetic variations in cervical cancer development. Here, we performed deep sequencing analyses of the viral genome to explore genetic variations of HPV16 that are prevalent in Japan. A total of 100 complete genome sequences of HPV16 were determined from cervical specimens collected from Japanese women with cervical intraepithelial neoplasia and invasive cervical cancer, or without cervical malignancies. Phylogenetic analyses revealed the variant distribution in the Japanese HPV16 isolates; overall, lineage A was the most prevalent (94.0%), in which sublineage A4 was dominant (52.0%), followed by sublineage A1 (21.0%). The relative risk of sublineage A4 for cervical cancer development was significantly higher compared to sublineages A1/A2/A3 (odds ratio = 6.72, 95% confidence interval = 1.78–28.9). Interestingly, a novel cluster of variants that branched from A1/A2/A3 was observed for the Japanese HPV16 isolates, indicating that unique HPV16 variants are prevalent among Japanese women. View Full-Text
Keywords: papillomavirus; cervical cancer; HPV16; variant papillomavirus; cervical cancer; HPV16; variant

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Hirose, Y.; Onuki, M.; Tenjimbayashi, Y.; Yamaguchi-Naka, M.; Mori, S.; Tasaka, N.; Satoh, T.; Morisada, T.; Iwata, T.; Kiyono, T.; Mimura, T.; Sekizawa, A.; Matsumoto, K.; Kukimoto, I. Whole-Genome Analysis of Human Papillomavirus Type 16 Prevalent in Japanese Women with or without Cervical Lesions. Viruses 2019, 11, 350.

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