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Open AccessArticle

A Novel Bacterium-Like Particle-Based Vaccine Displaying the SUDV Glycoprotein Induces Potent Humoral and Cellular Immune Responses in Mice

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College of Animal Science and Technology, Jilin Agricultural University, Changchun 130118, China
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Key Laboratory of Jilin Province for Zoonosis Prevention and Control, Military Veterinary Research Institute, Academy of Military Medical Sciences, Changchun 130122, China
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Key Laboratory of Zoonosis Research, Ministry of Education, College of Veterinary Medicine, Jilin University, Changchun 130062, China
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College of Veterinary Medicine, South China Agricultural University, Guangzhou 510642, China
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College of Wildlife and Protected Area, Northeast Forestry University, Harbin 150040, China
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Key Laboratory of Animal Resistant Biology of Shandong, Ruminant Diseases Research Center, College of Life Sciences, Shandong Normal University, Jinan 250014, China
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Jiangsu Co-innovation Center for Prevention and Control of Important Animal Infectious Diseases and Zoonoses, Yangzhou 225000, China
*
Authors to whom correspondence should be addressed.
Viruses 2019, 11(12), 1149; https://doi.org/10.3390/v11121149
Received: 23 October 2019 / Revised: 1 December 2019 / Accepted: 7 December 2019 / Published: 11 December 2019
(This article belongs to the Section Antivirals & Vaccines)
Sudan virus (SUDV) causes severe lethal hemorrhagic fever in humans and nonhuman primates. The most effective and economical way to protect against Sudan ebolavirus disease is prophylactic vaccination. However, there are no licensed vaccines to prevent SUDV infections. In this study, a bacterium-like particle (BLP)-based vaccine displaying the extracellular domain of the SUDV glycoprotein (eGP) was developed based on a gram-positive enhancer matrix-protein anchor (GEM-PA) surface display system. Expression of the recombinant GEM-displayed eGP (eGP-PA-GEM) was verified by Western blotting and immunofluorescence assays. The SUDV BLPs (SBLPs), which were mixed with Montanide ISA 201VG plus Poly (I:C) combined adjuvant, could induce high SUDV GP-specific IgG titers of up to 1:40,960 and robust virus-neutralizing antibody titers reached 1:460. The SBLP also elicited T-helper 1 (Th1) and T-helper 2 (Th2) cell-mediated immunity. These data indicate that the SBLP subunit vaccine has the potential to be developed into a promising candidate vaccine against SUDV infections. View Full-Text
Keywords: SUDV; subunit vaccine; bacterium-like particles; eGP; immune response SUDV; subunit vaccine; bacterium-like particles; eGP; immune response
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MDPI and ACS Style

Xu, S.; Jiao, C.; Jin, H.; Li, W.; Li, E.; Cao, Z.; Shi, Z.; Yan, F.; Zhang, S.; He, H.; Chi, H.; Feng, N.; Zhao, Y.; Gao, Y.; Yang, S.; Wang, J.; Wang, H.; Xia, X. A Novel Bacterium-Like Particle-Based Vaccine Displaying the SUDV Glycoprotein Induces Potent Humoral and Cellular Immune Responses in Mice. Viruses 2019, 11, 1149.

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