Next Article in Journal
Rapid, Unbiased PRRSV Strain Detection Using MinION Direct RNA Sequencing and Bioinformatics Tools
Previous Article in Journal
Recombinant Modified Vaccinia Virus Ankara (MVA) Vaccines Efficiently Protect Cockatiels Against Parrot Bornavirus Infection and Proventricular Dilatation Disease
Open AccessArticle

Pediatric Infections by Human mastadenovirus C Types 2, 89, and a Recombinant Type Detected in Japan between 2011 and 2018

1
Division 4, Infectious Disease Surveillance Center, National Institute of Infectious Diseases, Tokyo 162-8640, Japan
2
Department of Pediatrics, Tokyo Women’s Medical University Medical Center East, Tokyo 116-8567, Japan
3
Research Center for Zoonosis Control, Hokkaido University, Sapporo 001-0020, Japan
4
Kobayashi Pediatric Clinic, Fujieda 426-0067, Japan
5
National Virus Reference Laboratory, School of Medicine, University College Dublin, Dublin D04 V1W8, Ireland
6
Global Station for Zoonosis Control, Global Institution for Collaborative Research and Education (GI-CoRE), Hokkaido University, Sapporo 001-0020, Japan
7
Infectious Disease Research Division, Hyogo Prefectural Institute of Public Health Science, Kakogawa 675-0003, Japan
*
Author to whom correspondence should be addressed.
These authors contributed equally to this work.
Viruses 2019, 11(12), 1131; https://doi.org/10.3390/v11121131
Received: 22 November 2019 / Revised: 3 December 2019 / Accepted: 4 December 2019 / Published: 6 December 2019
(This article belongs to the Section Animal Viruses)
Between 2011 and 2018, 518 respiratory adenovirus infections were diagnosed in a pediatric clinic in Shizuoka, Japan. Detection and typing were performed by partial sequencing of both hexon- and fiber-coding regions which identified: adenovirus type 1 (Ad-1, n = 85), Ad-2 (n = 160), Ad-3 (n = 193), Ad-4 (n = 18), Ad-5 (n = 27), Ad-11 (n = 2), Ad-54 (n = 3), and Ad-56 (n = 1). Considering previous reports of the circulation of an endemic recombinant Ad-2, e.g., Ad-89, 100 samples typed as Ad-2 were randomly selected for further molecular typing by sequencing the penton base-coding region. Despite the high nucleotide sequence conservation in the penton base- coding region, 27 samples showed 98% identity to Ad-2. Furthermore, 14 samples showed 97.7% identity to Ad-2 and 99.8% identity to Ad-89, while the remaining 13 samples showed an average 98% pairwise identity to other Ad-C types and clustered with Ad-5. The samples typed as Ad-89 (n = 14) and as a recombinant Ad type (P5H2F2) (n = 13) represented 27% of cases originally diagnosed as Ad-2, and were detected sporadically. Therefore, two previously uncharacterized types in Japan, Ad-89 and a recombinant Ad-C, were shown to circulate in children. This study creates a precedent to evaluate the epidemiology and divergence among Ad-C types by comprehensively considering the type classification of adenoviruses. View Full-Text
Keywords: Human mastadenovirus C; respiratory infections; pediatric infections; recombination; molecular epidemiology; adenovirus typing Human mastadenovirus C; respiratory infections; pediatric infections; recombination; molecular epidemiology; adenovirus typing
Show Figures

Graphical abstract

MDPI and ACS Style

Takahashi, K.; Gonzalez, G.; Kobayashi, M.; Hanaoka, N.; Carr, M.J.; Konagaya, M.; Nojiri, N.; Ogi, M.; Fujimoto, T. Pediatric Infections by Human mastadenovirus C Types 2, 89, and a Recombinant Type Detected in Japan between 2011 and 2018. Viruses 2019, 11, 1131.

Show more citation formats Show less citations formats
Note that from the first issue of 2016, MDPI journals use article numbers instead of page numbers. See further details here.

Article Access Map by Country/Region

1
Search more from Scilit
 
Search
Back to TopTop