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Open AccessArticle

In Vitro and In Vivo Metabolomic Profiling after Infection with Virulent Newcastle Disease Virus

1
Shanghai Veterinary Research Institute, Chinese Academy of Agricultural Sciences, Shanghai 200241, China
2
College of Veterinary Medicine, Yangzhou University, Yangzhou 225009, Jiangsu, China
3
Jiangsu Co-innovation Center for Prevention and Control of Important Animal Infectious Diseases and Zoonoses, Yangzhou 225009, China
*
Authors to whom correspondence should be addressed.
Viruses 2019, 11(10), 962; https://doi.org/10.3390/v11100962
Received: 5 September 2019 / Revised: 14 October 2019 / Accepted: 15 October 2019 / Published: 18 October 2019
(This article belongs to the Section Animal Viruses)
Newcastle disease (ND) is an acute, febrile, highly contagious disease caused by the virulent Newcastle disease virus (vNDV). The disease causes serious economic losses to the poultry industry. However, the metabolic changes caused by vNDV infection remain unclear. The objective of this study was to determine the metabolomic profiling after infection with vNDV. DF-1 cells infected with the vNDV strain Herts/33 and the lungs from Herts/33-infected specific pathogen-free (SPF) chickens were analyzed via ultra-high-performance liquid chromatography/quadrupole time-of-flight tandem mass spectrometry (UHPLC-QTOF-MS) in combination with multivariate statistical analysis. A total of 305 metabolites were found to have changed significantly after Herts/33 infection, and most of them belong to the amino acid and nucleotide metabolic pathway. It is suggested that the increased pools of amino acids and nucleotides may benefit viral protein synthesis and genome amplification to promote NDV infection. Similar results were also confirmed in vivo. Identification of these metabolites will provide information to further understand the mechanism of vNDV replication and pathogenesis. View Full-Text
Keywords: virulent Newcastle disease virus; metabolomic analysis; in vitro and in vivo; UHPLC-QTOF-MS virulent Newcastle disease virus; metabolomic analysis; in vitro and in vivo; UHPLC-QTOF-MS
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Liu, P.; Yin, Y.; Gong, Y.; Qiu, X.; Sun, Y.; Tan, L.; Song, C.; Liu, W.; Liao, Y.; Meng, C.; Ding, C. In Vitro and In Vivo Metabolomic Profiling after Infection with Virulent Newcastle Disease Virus. Viruses 2019, 11, 962.

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