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Viruses 2018, 10(3), 109; https://doi.org/10.3390/v10030109

Molecular Requirements for Self-Interaction of the Respiratory Syncytial Virus Matrix Protein in Living Mammalian Cells

1
Department of Molecular Medicine, University of Padua, Padua 35121, Italy
2
Department of Molecular and Translational Medicine, University of Brescia, Brescia 25123, Italy
3
Centre for Research in Therapeutic Solutions, Faculty of Science and Technology, University of Canberra, Canberra 2617, Australia
These authors contributed equally to this work.
*
Authors to whom correspondence should be addressed.
Received: 9 February 2018 / Revised: 26 February 2018 / Accepted: 28 February 2018 / Published: 3 March 2018
(This article belongs to the Section Animal Viruses)
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Abstract

Respiratory syncytial virus (RSV) is an important human pathogen, which infects respiratory tract epithelial cells causing bronchiolitis and pneumonia in children and the elderly. Recent studies have linked RSV matrix (M) ability to self-interaction and viral budding. However, RSV M has been crystalized both as a monomer and a dimer, and no formal proof exists to date that it forms dimers in cells. Here, by using a combination of confocal laser scanning microscopy and bioluminescent resonant energy transfer applied to differently tagged deletion mutants of RSV M, we show that the protein can self-interact in living mammalian cells and that both the N and C-terminus of the protein are strictly required for the process, consistent with the reported dimeric crystal structure. View Full-Text
Keywords: RSV M protein; virus assembly; Bioluminescence resonance energy transfer (BRET); confocal microscopy RSV M protein; virus assembly; Bioluminescence resonance energy transfer (BRET); confocal microscopy
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Trevisan, M.; Di Antonio, V.; Radeghieri, A.; Palù, G.; Ghildyal, R.; Alvisi, G. Molecular Requirements for Self-Interaction of the Respiratory Syncytial Virus Matrix Protein in Living Mammalian Cells. Viruses 2018, 10, 109.

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