Viruses 2009, 1(3), 420-440; https://doi.org/10.3390/v1030420
Dominant-Negative Proteins in Herpesviruses – From Assigning Gene Function to Intracellular Immunization
Max-von-Pettenkofer Institut, LMU, Feodor-Lynenstr. 25, 81377 Munich, Germany
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These authors contributed equally to this work.
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Received: 18 August 2009 / Revised: 19 October 2009 / Accepted: 19 October 2009 / Published: 19 October 2009
(This article belongs to the Special Issue Antiviral Responses to Herpes Viruses)
Abstract
Investigating and assigning gene functions of herpesviruses is a process, which profits from consistent technical innovation. Cloning of bacterial artificial chromosomes encoding herpesvirus genomes permits nearly unlimited possibilities in the construction of genetically modified viruses. Targeted or randomized screening approaches allow rapid identification of essential viral proteins. Nevertheless, mapping of essential genes reveals only limited insight into function. The usage of dominant-negative (DN) proteins has been the tool of choice to dissect functions of proteins during the viral life cycle. DN proteins also facilitate the analysis of host-virus interactions. Finally, DNs serve as starting-point for design of new antiviral strategies. View Full-TextKeywords:
dominant-negative; essential genes; random mutagenesis; conditional gene expression; deletion; intracellular immunization; herpesvirus; conserved gene blocks
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Mühlbach, H.; Mohr, C.A.; Ruzsics, Z.; Koszinowski, U.H. Dominant-Negative Proteins in Herpesviruses – From Assigning Gene Function to Intracellular Immunization. Viruses 2009, 1, 420-440.