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Article

Influence of 3D Printing Parameters on the Mechanical Stability of PCL Scaffolds and the Proliferation Behavior of Bone Cells

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G.E.R.N. Center of Tissue Replacement, Regeneration & Neogenesis, Department of Orthopedics and Trauma Surgery, Medical Center—Albert-Ludwigs-University of Freiburg, Faculty of Medicine, Albert-Ludwigs-University of Freiburg, Hugstetter Straße 55, 79106 Freiburg, Germany
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Institute for Applied Biomechanics, Offenburg University, Badstraße 24, 77652 Offenburg, Germany
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Author to whom correspondence should be addressed.
Academic Editor: Young-Hag Koh
Materials 2022, 15(6), 2091; https://doi.org/10.3390/ma15062091
Received: 24 January 2022 / Revised: 8 March 2022 / Accepted: 9 March 2022 / Published: 11 March 2022
Introduction The use of scaffolds in tissue engineering is becoming increasingly important as solutions need to be found for the problem of preserving human tissue, such as bone or cartilage. In this work, scaffolds were printed from the biomaterial known as polycaprolactone (PCL) on a 3D Bioplotter. Both the external and internal geometry were varied to investigate their influence on mechanical stability and biocompatibility. Materials and Methods: An Envisiontec 3D Bioplotter was used to fabricate the scaffolds. First, square scaffolds were printed with variations in the strand width and strand spacing. Then, the filling structure was varied: either lines, waves, and honeycombs were used. This was followed by variation in the outer shape, produced as either a square, hexagon, octagon, or circle. Finally, the internal and external geometry was varied. To improve interaction with the cells, the printed PCL scaffolds were coated with type-I collagen. MG-63 cells were then cultured on the scaffolds and various tests were performed to investigate the biocompatibility of the scaffolds. Results: With increasing strand thickness and strand spacing, the compressive strengths decreased from 86.18 + 2.34 MPa (200 µm) to 46.38 + 0.52 MPa (600 µm). The circle was the outer shape with the highest compressive strength of 76.07 + 1.49 MPa, compared to the octagon, which had the lowest value of 52.96 ± 0.98 MPa. Varying the external shape (toward roundness) geometry, as well as the filling configuration, resulted in the highest values of compressive strength for the round specimens with honeycomb filling, which had a value of 91.4 + 1.4 MPa. In the biocompatibility tests, the round specimens with honeycomb filling also showed the highest cell count per mm2, with 1591 ± 239 live cells/mm2 after 10 days and the highest value in cell proliferation, but with minimal cytotoxic effects (9.19 ± 2.47% after 3 days). View Full-Text
Keywords: 3D printing; PCL scaffold; geometry variation; mechanical properties; biocompatibility 3D printing; PCL scaffold; geometry variation; mechanical properties; biocompatibility
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MDPI and ACS Style

Huber, F.; Vollmer, D.; Vinke, J.; Riedel, B.; Zankovic, S.; Schmal, H.; Seidenstuecker, M. Influence of 3D Printing Parameters on the Mechanical Stability of PCL Scaffolds and the Proliferation Behavior of Bone Cells. Materials 2022, 15, 2091. https://doi.org/10.3390/ma15062091

AMA Style

Huber F, Vollmer D, Vinke J, Riedel B, Zankovic S, Schmal H, Seidenstuecker M. Influence of 3D Printing Parameters on the Mechanical Stability of PCL Scaffolds and the Proliferation Behavior of Bone Cells. Materials. 2022; 15(6):2091. https://doi.org/10.3390/ma15062091

Chicago/Turabian Style

Huber, Fabian, David Vollmer, Johannes Vinke, Bianca Riedel, Sergej Zankovic, Hagen Schmal, and Michael Seidenstuecker. 2022. "Influence of 3D Printing Parameters on the Mechanical Stability of PCL Scaffolds and the Proliferation Behavior of Bone Cells" Materials 15, no. 6: 2091. https://doi.org/10.3390/ma15062091

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