Next Article in Journal
Enhancing Photovoltaic Performance of Plasmonic Silicon Solar Cells with ITO Nanoparticles Dispersed in SiO2 Anti-Reflective Layer
Previous Article in Journal
Industrial Ceramic Brick Drying in Oven by CFD
Article Menu

Export Article

Open AccessArticle

Evaluation of the Bone Regeneration Effect of Recombinant Human Bone Morphogenic Protein-2 on Subperiosteal Bone Graft in the Rat Calvarial Model

1
Department of Orthodontics, College of Dentistry, Gangneung-Wonju National University, Gangneung 25457, Korea
2
Department of Prosthodontics, Chungbuk National University Hospital, Cheongju 28644, Korea
3
Department of Oral and Maxillofacial Surgery, Chungbuk National University College of Medicine, Cheongju 28644, Korea
4
Department of Oral and Maxillofacial Surgery, Chungbuk National University Hospital, Cheongju 28644, Korea
*
Author to whom correspondence should be addressed.
Materials 2019, 12(10), 1613; https://doi.org/10.3390/ma12101613
Received: 2 May 2019 / Revised: 10 May 2019 / Accepted: 13 May 2019 / Published: 16 May 2019
(This article belongs to the Section Biomaterials)
  |  
PDF [4210 KB, uploaded 16 May 2019]
  |     |  

Abstract

The aim of this study was to evaluate the bone regeneration effect of recombinant human bone morphogenetic protein-2 (rhBMP-2) on a subperiosteal bone graft in a rat model. A subperiosteal space was made on the rat calvarium, and anorganic bovine bone (ABB), ABB/low bone morphogenetic protein (BMP) (5 µg), and ABB/high BMP (50 µg) were grafted as subperiosteal bone grafts. The new bone formation parameters of bone volume (BV), bone mineral density (BMD), trabecular thickness (TbTh), and trabecular spacing (TbSp) were evaluated by microcomputed tomography (µ-CT), and a histomorphometric analysis was performed to evaluate the new bone formation area. The expression of osteogenic markers, such as bone sialoprotein (BSP) and osteocalcin, were evaluated by immunohistochemistry (IHC). The ABB/high BMP group showed significantly higher BV than the ABB/low BMP (p = 0.004) and control groups (p = 0.000) and higher TbTh than the control group (p = 0.000). The ABB/low BMP group showed significantly higher BV, BMD, and TbTh than the control group (p = 0.002, 0.042, and 0.000, respectively). The histomorphometry showed significantly higher bone formation in the ABB/low and high BMP groups than in the control group (p = 0.000). IHC showed a high expression of BSP and osteocalcin in the ABB/low and high BMP groups. Subperiosteal bone grafts with ABB and rhBMP-2 have not been studied. In our study, we confirmed that rhBMP-2 contributes to new bone formation in a subperiosteal bone graft with ABB. View Full-Text
Keywords: bone morphogenetic protein; bone regeneration; subperiosteal bone graft; bone sialoprotein; osteocalcin bone morphogenetic protein; bone regeneration; subperiosteal bone graft; bone sialoprotein; osteocalcin
Figures

Figure 1

This is an open access article distributed under the Creative Commons Attribution License which permits unrestricted use, distribution, and reproduction in any medium, provided the original work is properly cited (CC BY 4.0).
SciFeed

Share & Cite This Article

MDPI and ACS Style

Jang, E.; Lee, J.-Y.; Lee, E.-Y.; Seok, H. Evaluation of the Bone Regeneration Effect of Recombinant Human Bone Morphogenic Protein-2 on Subperiosteal Bone Graft in the Rat Calvarial Model. Materials 2019, 12, 1613.

Show more citation formats Show less citations formats

Note that from the first issue of 2016, MDPI journals use article numbers instead of page numbers. See further details here.

Related Articles

Article Metrics

Article Access Statistics

1

Comments

[Return to top]
Materials EISSN 1996-1944 Published by MDPI AG, Basel, Switzerland RSS E-Mail Table of Contents Alert
Back to Top