Next Article in Journal
Methodology for the Energy Need Assessment to Effectively Design and Deploy Mini-Grids for Rural Electrification
Next Article in Special Issue
A Review on Anaerobic Co-Digestion with a Focus on the Microbial Populations and the Effect of Multi-Stage Digester Configuration
Previous Article in Journal
Influence of Disc Tip Geometry on the Aerodynamic Performance and Flow Characteristics of Multichannel Tesla Turbines
Article Menu
Issue 3 (February-1) cover image

Export Article

Open AccessArticle
Energies 2019, 12(3), 573; https://doi.org/10.3390/en12030573

Performance and Microbial Community Dynamics in Anaerobic Digestion of Waste Activated Sludge: Impact of Immigration

1
Department of Energy Engineering, Future Convergence Technology Research Institute, Gyeongnam National University of Science and Technology, Jinju, Gyeongnam 52725, South Korea
2
Department of Biological and Environmental Science, Dongguk University, Goyang, Gyeonggi-do 10326, South Korea
3
Division of Environmental Science and Engineering, Pohang University of Science and Technology, Pohang, Gyeongbuk 37673, South Korea
4
Environmental Process Engineering Team, Global Engineering Division, GS E&C, Seoul 03159, South Korea
5
Department of Environmental Engineering, Gyeongnam National University of Science and Technology, Jinju, Gyeongnam 52725, South Korea
*
Author to whom correspondence should be addressed.
These authors contributed equally to this work.
Received: 8 January 2019 / Revised: 2 February 2019 / Accepted: 5 February 2019 / Published: 12 February 2019
(This article belongs to the Special Issue Anaerobic Digestion for the Production of Energy and Chemicals)
Full-Text   |   PDF [3327 KB, uploaded 12 February 2019]   |  

Abstract

Waste activated sludge (WAS) is a byproduct of municipal wastewater treatment. WAS contains a large proportion of inactive microbes, so when it is used as a substrate for anaerobic digestion (AD), their presence can interfere with monitoring of active microbial populations. To investigate how influent cells affect the active and inactive microbial communities during digestion of WAS, we operated model mesophilic bioreactors with conventional conditions. Under six different hydraulic retention times (HRTs; 25, 23, 20, 17, 14, and 11.5 d), the chemical oxygen demand (COD) removal and CH4 production of the AD were within a typical range for mesophilic sludge digesters. In the main bacteria were proteobacteria, bacteroidetes, and firmicutes in both the WAS and the bioreactors, while in main archaeal methanogen group was Methanosarcinales in the WAS and methanomicrobiales in the bioreactors. Of the 106 genera identified, the estimated net growth rates were negative in 72 and positive in 34. The genera with negative growth included many aerobic taxa. The genera with positive growth rates included methanogens and syntrophs. In some taxa, the net growth rate could be positive or negative, depending on HRT, so their abundance was also affected by HRT. This study gives insights into the microbial dynamics of a conventional sludge anaerobic digester by distinguishing potentially active (growing) and inactive (non-growing, dormant) microbes and by correlating population dynamics with process parameters. View Full-Text
Keywords: sewage sludge; hydraulic retention time; high-throughput sequencing; real-time PCR; net growth rate sewage sludge; hydraulic retention time; high-throughput sequencing; real-time PCR; net growth rate
Figures

Figure 1

This is an open access article distributed under the Creative Commons Attribution License which permits unrestricted use, distribution, and reproduction in any medium, provided the original work is properly cited (CC BY 4.0).
SciFeed

Share & Cite This Article

MDPI and ACS Style

Shin, J.; Cho, S.-K.; Lee, J.; Hwang, K.; Chung, J.W.; Jang, H.-N.; Shin, S.G. Performance and Microbial Community Dynamics in Anaerobic Digestion of Waste Activated Sludge: Impact of Immigration. Energies 2019, 12, 573.

Show more citation formats Show less citations formats

Note that from the first issue of 2016, MDPI journals use article numbers instead of page numbers. See further details here.

Related Articles

Article Metrics

Article Access Statistics

1

Comments

[Return to top]
Energies EISSN 1996-1073 Published by MDPI AG, Basel, Switzerland RSS E-Mail Table of Contents Alert
Back to Top