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Article

The Acute Effects of Exercise and Temperature on Regional mtDNA

1
School of Health and Kinesiology, University of Nebraska at Omaha, Omaha, NE 68182, USA
2
School of Integrative Physiology and Athletic Training, University of Montana, Missoula, MT 59812, USA
*
Author to whom correspondence should be addressed.
Academic Editors: Heidi K. Ortmeyer and Paul B. Tchounwou
Int. J. Environ. Res. Public Health 2021, 18(12), 6382; https://doi.org/10.3390/ijerph18126382
Received: 4 May 2021 / Revised: 2 June 2021 / Accepted: 10 June 2021 / Published: 12 June 2021
A reduced mitochondrial DNA (mtDNA) copy number, the ratio of mitochondrial DNA to genomic DNA (mtDNA:gDNA), has been linked with dysfunctional mitochondria. Exercise can acutely induce mtDNA damage manifested as a reduced copy number. However, the influence of a paired (exercise and temperature) intervention on regional mtDNA (MINor Arc and MAJor Arc) are unknown. Thus, the purpose of this study was to determine the acute effects of exercise in cold (7 °C), room temperature (20 °C), and hot (33 °C) ambient temperatures, on regional mitochondrial copy number (MINcn and MAJcn). Thirty-four participants (24.4 ± 5.1 yrs, 87.1 ± 22.1 kg, 22.3 ± 8.5 %BF, and 3.20 ± 0.59 L·min−1 VO2peak) cycled for 1 h (261.1 ± 22.1 W) in either 7 °C, 20 °C, or 33 °C ambient conditions. Muscle biopsy samples were collected from the vastus lateralis to determine mtDNA regional copy numbers via RT-qPCR. mtDNA is sensitive to the stressors of exercise post-exercise (MIN fold change, −1.50 ± 0.11; MAJ fold change, −1.70 ± 0.12) and 4-h post-exercise (MIN fold change, −0.82 ± 0.13; MAJ fold change, −1.54 ± 0.11). The MAJ Arc seems to be more sensitive to heat, showing a temperature-trend (p = 0.056) for a reduced regional copy number ratio after exercise in the heat (fold change −2.81 ± 0.11; p = 0.019). These results expand upon our current knowledge of the influence of temperature and exercise on the acute remodeling of regional mtDNA. View Full-Text
Keywords: mitochondria; thermoregulation; mtDNA; exercise; copy number; ambient temperature mitochondria; thermoregulation; mtDNA; exercise; copy number; ambient temperature
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MDPI and ACS Style

McGlynn, M.L.; Schnitzler, H.; Shute, R.; Ruby, B.; Slivka, D. The Acute Effects of Exercise and Temperature on Regional mtDNA. Int. J. Environ. Res. Public Health 2021, 18, 6382. https://doi.org/10.3390/ijerph18126382

AMA Style

McGlynn ML, Schnitzler H, Shute R, Ruby B, Slivka D. The Acute Effects of Exercise and Temperature on Regional mtDNA. International Journal of Environmental Research and Public Health. 2021; 18(12):6382. https://doi.org/10.3390/ijerph18126382

Chicago/Turabian Style

McGlynn, Mark L., Halee Schnitzler, Robert Shute, Brent Ruby, and Dustin Slivka. 2021. "The Acute Effects of Exercise and Temperature on Regional mtDNA" International Journal of Environmental Research and Public Health 18, no. 12: 6382. https://doi.org/10.3390/ijerph18126382

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