Next Article in Journal
Microbial Oligosaccharides with Biomedical Applications
Next Article in Special Issue
Transcriptomics and Metabolomics Analyses Provide Novel Insights into Glucose-Induced Trophic Transition of the Marine Diatom Nitzschia laevis
Previous Article in Journal
Four New Chromones from the Endophytic Fungus Phomopsis asparagi DHS-48 Isolated from the Chinese Mangrove Plant Rhizophora mangle
Previous Article in Special Issue
NaCl Promotes the Efficient Formation of Haematococcus pluvialis Nonmotile Cells under Phosphorus Deficiency
Article

Distribution of the Water-Soluble Astaxanthin Binding Carotenoprotein (AstaP) in Scenedesmaceae

1
Department of Bioscience, Tokyo University of Agriculture, 1-1-1 Sakuragaoka, Setagaya-ku, Tokyo 156-8502, Japan
2
NODAI Genome Research Centre, Tokyo University of Agriculture, 1-1-1 Sakuragaoka, Setagaya-ku, Tokyo 156-8502, Japan
3
Department of Molecular Microbiology, Tokyo University of Agriculture, 1-1-1 Sakuragaoka, Setagaya-ku, Tokyo 156-8502, Japan
*
Author to whom correspondence should be addressed.
Academic Editors: Masashi Hosokawa and Hayato Maeda
Mar. Drugs 2021, 19(6), 349; https://doi.org/10.3390/md19060349
Received: 19 May 2021 / Revised: 17 June 2021 / Accepted: 18 June 2021 / Published: 20 June 2021
Photooxidative stress-inducible water-soluble astaxanthin-binding proteins, designated as AstaP, were identified in two Scenedesmaceae strains, Coelastrella astaxanthina Ki-4 and Scenedesmus obtusus Oki-4N; both strains were isolated under high light conditions. These AstaPs are classified as a novel family of carotenoprotein and are useful for providing valuable astaxanthin in water-soluble form; however, the distribution of AstaP orthologs in other microalgae remains unknown. Here, we examined the distribution of AstaP orthologs in the family Scenedesmaceae with two model microalgae, Chlamydomonas reinhardtii and Chlorella variabilis. The expression of AstaP orthologs under photooxidative stress conditions was detected in cell extracts of Scenedesmaceae strains, but not in model algal strains. Aqueous orange proteins produced by Scenedesmaceae strains were shown to bind astaxanthin. The protein from Scenedesmus costatus SAG 46.88 was purified. It was named ScosAstaP and found to bind astaxanthin. The deduced amino acid sequence from a gene encoding ScosAstaP showed 62% identity to Ki-4 AstaP. The expression of the genes encoding AstaP orthologs was shown to be inducible under photooxidative stress conditions; however, the production amounts of AstaP orthologs were estimated to be approximately 5 to 10 times lower than that of Ki-4 and Oki-4N. View Full-Text
Keywords: astaxanthin; Scenedesmus; Coelastrella; carotenoprotein; astaxanthin-binding protein astaxanthin; Scenedesmus; Coelastrella; carotenoprotein; astaxanthin-binding protein
Show Figures

Figure 1

MDPI and ACS Style

Toyoshima, H.; Miyata, A.; Yoshida, R.; Ishige, T.; Takaichi, S.; Kawasaki, S. Distribution of the Water-Soluble Astaxanthin Binding Carotenoprotein (AstaP) in Scenedesmaceae. Mar. Drugs 2021, 19, 349. https://doi.org/10.3390/md19060349

AMA Style

Toyoshima H, Miyata A, Yoshida R, Ishige T, Takaichi S, Kawasaki S. Distribution of the Water-Soluble Astaxanthin Binding Carotenoprotein (AstaP) in Scenedesmaceae. Marine Drugs. 2021; 19(6):349. https://doi.org/10.3390/md19060349

Chicago/Turabian Style

Toyoshima, Hiroki, Ami Miyata, Risako Yoshida, Taichiro Ishige, Shinichi Takaichi, and Shinji Kawasaki. 2021. "Distribution of the Water-Soluble Astaxanthin Binding Carotenoprotein (AstaP) in Scenedesmaceae" Marine Drugs 19, no. 6: 349. https://doi.org/10.3390/md19060349

Find Other Styles
Note that from the first issue of 2016, MDPI journals use article numbers instead of page numbers. See further details here.

Article Access Map by Country/Region

1
Back to TopTop