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Article

Gut Organoid as a New Platform to Study Alginate and Chitosan Mediated PLGA Nanoparticles for Drug Delivery

1
Department of Chemical and Biological Engineering, Iowa State University, Ames, IA 50011, USA
2
Department of Veterinary Microbiology and Preventive Medicine, Iowa State University, Ames, IA 50011, USA
3
Department of Veterinary Clinical Sciences, Iowa State University, Ames, IA 50011, USA
*
Author to whom correspondence should be addressed.
Mar. Drugs 2021, 19(5), 282; https://doi.org/10.3390/md19050282
Received: 27 April 2021 / Revised: 17 May 2021 / Accepted: 18 May 2021 / Published: 20 May 2021
Intestinal organoids can be used as an ex vivo epithelial model to study different drug delivery effects on epithelial cells’ luminal surface. In this study, the impact of surface charge on the delivery of 5-ASA loaded PLGA nanoparticles into the lumen of organoids was investigated. Alginate and chitosan were used to coat the nanoparticles and provide negative and positive charges on the particles, respectively. The organoid growth and viability were not affected by the presence of either alginate- or chitosan-coated nanoparticles. It was shown that nanoparticles could be transported from the serosal side of the organoids to the lumen as the dye gradually accumulated in the lumen by day 2–3 after adding the nanoparticles to the Matrigel. By day 5, the dye was eliminated from the lumen of the organoids. It was concluded that the positively charged nanoparticles were more readily transported across the epithelium into the lumen. It may be attributed to the affinity of epithelial cells to the positive charge. Thus, the organoid can be utilized as an appropriate model to mimic the functions of the intestinal epithelium and can be used as a model to evaluate the benefits of nanoparticle-based drug delivery. View Full-Text
Keywords: organoids; alginate; chitosan; PLGA; nanoparticles organoids; alginate; chitosan; PLGA; nanoparticles
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MDPI and ACS Style

Davoudi, Z.; Peroutka-Bigus, N.; Bellaire, B.; Jergens, A.; Wannemuehler, M.; Wang, Q. Gut Organoid as a New Platform to Study Alginate and Chitosan Mediated PLGA Nanoparticles for Drug Delivery. Mar. Drugs 2021, 19, 282. https://doi.org/10.3390/md19050282

AMA Style

Davoudi Z, Peroutka-Bigus N, Bellaire B, Jergens A, Wannemuehler M, Wang Q. Gut Organoid as a New Platform to Study Alginate and Chitosan Mediated PLGA Nanoparticles for Drug Delivery. Marine Drugs. 2021; 19(5):282. https://doi.org/10.3390/md19050282

Chicago/Turabian Style

Davoudi, Zahra, Nathan Peroutka-Bigus, Bryan Bellaire, Albert Jergens, Michael Wannemuehler, and Qun Wang. 2021. "Gut Organoid as a New Platform to Study Alginate and Chitosan Mediated PLGA Nanoparticles for Drug Delivery" Marine Drugs 19, no. 5: 282. https://doi.org/10.3390/md19050282

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