Nostoc edaphicum CCNP1411 from the Baltic Sea—A New Producer of Nostocyclopeptides
1
Division of Marine Biotechnology, Faculty of Oceanography and Geography, University of Gdańsk, Marszałka J. Piłsudskiego 46, PL-81378 Gdynia, Poland
2
Department of Molecular Biology, University of Gdansk, Wita Stwosza 59, 80-308 Gdansk, Poland
3
DNA Sequencing and Oligonucleotide Synthesis Laboratory, Polish Academy of Sciences, Institute of Biochemistry and Biophysics, 02-106 Warsaw, Poland
*
Author to whom correspondence should be addressed.
Mar. Drugs 2020, 18(9), 442; https://doi.org/10.3390/md18090442
Received: 31 July 2020
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Revised: 21 August 2020
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Accepted: 25 August 2020
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Published: 26 August 2020
(This article belongs to the Special Issue Bioactive Molecules from Marine Microorganisms)
Nostocyclopeptides (Ncps) constitute a small class of nonribosomal peptides, exclusively produced by cyanobacteria of the genus Nostoc. The peptides inhibit the organic anion transporters, OATP1B3 and OATP1B1, and prevent the transport of the toxic microcystins and nodularin into hepatocytes. So far, only three structural analogues, Ncp-A1, Ncp-A2 and Ncp-M1, and their linear forms were identified in Nostoc strains as naturally produced cyanometabolites. In the current work, the whole genome sequence of the new Ncps producer, N. edaphicum CCNP1411 from the Baltic Sea, has been determined. The genome consists of the circular chromosome (7,733,505 bps) and five circular plasmids (from 44.5 kb to 264.8 kb). The nostocyclopeptide biosynthetic gene cluster (located between positions 7,609,981–7,643,289 bps of the chromosome) has been identified and characterized in silico. The LC-MS/MS analyzes of N. edaphicum CCNP1411 cell extracts prepared in aqueous methanol revealed several products of the genes. Besides the known peptides, Ncp-A1 and Ncp-A2, six other compounds putatively characterized as new noctocyclopeptide analogues were detected. This includes Ncp-E1 and E2 and their linear forms (Ncp-E1-L and E2-L), a cyclic Ncp-E3 and a linear Ncp-E4-L. Regardless of the extraction conditions, the cell contents of the linear nostocyclopeptides were found to be higher than the cyclic ones, suggesting a slow rate of the macrocyclization process.
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Keywords:
cyanobacteria; nostocyclopeptides; Nostoc; ncp gene cluster; nonribosomal peptide synthetase
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MDPI and ACS Style
Fidor, A.; Grabski, M.; Gawor, J.; Gromadka, R.; Węgrzyn, G.; Mazur-Marzec, H. Nostoc edaphicum CCNP1411 from the Baltic Sea—A New Producer of Nostocyclopeptides. Mar. Drugs 2020, 18, 442. https://doi.org/10.3390/md18090442
AMA Style
Fidor A, Grabski M, Gawor J, Gromadka R, Węgrzyn G, Mazur-Marzec H. Nostoc edaphicum CCNP1411 from the Baltic Sea—A New Producer of Nostocyclopeptides. Marine Drugs. 2020; 18(9):442. https://doi.org/10.3390/md18090442
Chicago/Turabian StyleFidor, Anna, Michał Grabski, Jan Gawor, Robert Gromadka, Grzegorz Węgrzyn, and Hanna Mazur-Marzec. 2020. "Nostoc edaphicum CCNP1411 from the Baltic Sea—A New Producer of Nostocyclopeptides" Marine Drugs 18, no. 9: 442. https://doi.org/10.3390/md18090442
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