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Open AccessArticle

A Novel High-Throughput Screening Platform Identifies Itaconate Derivatives from Marine Penicillium antarcticum as Inhibitors of Mesenchymal Stem Cell Differentiation

1
Regenerative Medicine Institute, School of Medicine, National University of Ireland Galway, H91TK33 Galway, Ireland
2
Marine Biodiscovery, School of Chemistry and Ryan Institute, National University of Ireland Galway, H91TK33 Galway, Ireland
3
Genomics and Screening Core, National Centre for Biomedical Engineering Science, National University of Ireland Galway, H91TK33 Galway, Ireland
4
Pharmacology and Therapeutics, School of Medicine, National University of Ireland Galway, H91TK33 Galway, Ireland
5
Molecular Glycobiotechnology, School of Natural Sciences, National University of Ireland Galway, H91TK33 Galway, Ireland
6
Galway University Hospital, National University of Ireland Galway, School of Medicine, H91YR71 Galway, Ireland
*
Author to whom correspondence should be addressed.
Mar. Drugs 2020, 18(4), 192; https://doi.org/10.3390/md18040192
Received: 20 February 2020 / Revised: 2 April 2020 / Accepted: 4 April 2020 / Published: 5 April 2020
(This article belongs to the Special Issue High-Throughput Screening of Marine Resources)
Worldwide diffused diseases such as osteoarthritis, atherosclerosis or chronic kidney disease are associated with a tissue calcification process which may involve unexpected local stem cell differentiation. Current pharmacological treatments for such musculoskeletal conditions are weakly effective, sometimes extremely expensive and often absent. The potential to develop new therapies is represented by the discovery of small molecules modulating resident progenitor cell differentiation to prevent aberrant tissue calcification. The marine environment is a rich reserve of compounds with pharmaceutical potential and many novel molecules are isolated from macro and microorganisms annually. The potential of small molecules synthetized by marine filamentous fungi to influence the osteogenic and chondrogenic differentiation of human mesenchymal stem/stromal cells (hMSCs) was investigated using a novel, high-throughput automated screening platform. Metabolites synthetized by the marine-derived fungus Penicillium antarcticum were evaluated on the platform. Itaconic acid derivatives were identified as inhibitors of calcium elaboration into the matrix of osteogenically differentiated hMSCs and also inhibited hMSC chondrogenic differentiation, highlighting their capacity to impair ectopic calcification. Bioactive small molecule discovery is critical to address ectopic tissue calcification and the use of biologically relevant assays to identify naturally occurring metabolites from marine sources represents a strategy that can contribute to this effort. View Full-Text
Keywords: marine fungi; high throughput screening; stem cell; regenerative medicine; drug discovery; Penicillium antarcticum marine fungi; high throughput screening; stem cell; regenerative medicine; drug discovery; Penicillium antarcticum
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MDPI and ACS Style

Marchese, P.; Mahajan, N.; O’Connell, E.; Fearnhead, H.; Tuohy, M.; Krawczyk, J.; Thomas, O.P.; Barry, F.; Murphy, M.J. A Novel High-Throughput Screening Platform Identifies Itaconate Derivatives from Marine Penicillium antarcticum as Inhibitors of Mesenchymal Stem Cell Differentiation. Mar. Drugs 2020, 18, 192. https://doi.org/10.3390/md18040192

AMA Style

Marchese P, Mahajan N, O’Connell E, Fearnhead H, Tuohy M, Krawczyk J, Thomas OP, Barry F, Murphy MJ. A Novel High-Throughput Screening Platform Identifies Itaconate Derivatives from Marine Penicillium antarcticum as Inhibitors of Mesenchymal Stem Cell Differentiation. Marine Drugs. 2020; 18(4):192. https://doi.org/10.3390/md18040192

Chicago/Turabian Style

Marchese, Pietro; Mahajan, Nipun; O’Connell, Enda; Fearnhead, Howard; Tuohy, Maria; Krawczyk, Janusz; Thomas, Olivier P.; Barry, Frank; Murphy, Mary J. 2020. "A Novel High-Throughput Screening Platform Identifies Itaconate Derivatives from Marine Penicillium antarcticum as Inhibitors of Mesenchymal Stem Cell Differentiation" Mar. Drugs 18, no. 4: 192. https://doi.org/10.3390/md18040192

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