Next Article in Journal
Biosynthesis of Saxitoxin in Marine Dinoflagellates: An Omics Perspective
Previous Article in Journal
Nutritional Profiling and the Value of Processing By-Products from Gilthead Sea Bream (Sparus aurata)
Previous Article in Special Issue
Genome-Wide Identification and Characterization of SODs in Zhikong Scallop Reveals Gene Expansion and Regulation Divergence after Toxic Dinoflagellate Exposure
Open AccessArticle

A Truncated Galectin-3 Isolated from Skin Mucus of Atlantic Salmon Salmo salar Binds to and Modulates the Proteome of the Gram-Negative Bacteria Moritella viscosa

1
Faculty of Biosciences and Aquaculture, Nord University, 8049 Bodø, Norway
2
Noto Marine Laboratory, Division of Marine Environmental Studies, Institute of Nature and Environmental Technology, Kanazawa University, Noto-Cho, Ishikawa 927-0553, Japan
*
Author to whom correspondence should be addressed.
Mar. Drugs 2020, 18(2), 102; https://doi.org/10.3390/md18020102
Received: 22 December 2019 / Revised: 31 January 2020 / Accepted: 2 February 2020 / Published: 4 February 2020
(This article belongs to the Special Issue Chemical Defense in Marine Organisms)
The mucus of fish skin plays a vital role in innate immune defense. Some mucus proteins have the potential to incapacitate pathogens and/or inhibit their passage through the skin. In this study the aim was to isolate and characterize galectin(s), β-galactosides binding proteins, present in skin mucus. A novel short form of galectin-3 was isolated from Atlantic salmon skin mucus by α-lactose agarose based affinity chromatography followed by Sephadex G-15 gel filtration. Mass spectrometric analysis showed that the isolated protein was the C-terminal half of galectin-3 (galectin-3C). Galectin-3C showed calcium independent and lactose inhabitable hemagglutination, and agglutinated the Gram-negative pathogenic bacteria Moritella viscosa. Galectin-3 mRNA was highly expressed in skin and gill, followed by muscle, hindgut, spleen, stomach, foregut, head kidney, and liver. Moritella viscosa incubated with galectin-3C had a modified proteome. Proteins with changed abundance included multidrug transporter and three ribosomal proteins L7/12, S2, and S13. Overall, this study shows the isolation and characterization of a novel galectin-3 short form involved in pathogen recognition and modulation, and hence in immune defense of Atlantic salmon. View Full-Text
Keywords: galectin-3; bacteria; proteomics; Gram-negative; lectin; innate immunology; mucosal immunology; agglutination; hemagglutination; multidrug transporter galectin-3; bacteria; proteomics; Gram-negative; lectin; innate immunology; mucosal immunology; agglutination; hemagglutination; multidrug transporter
Show Figures

Figure 1

MDPI and ACS Style

Patel, D.M.; Kitani, Y.; Korsnes, K.; Iversen, M.H.; Brinchmann, M.F. A Truncated Galectin-3 Isolated from Skin Mucus of Atlantic Salmon Salmo salar Binds to and Modulates the Proteome of the Gram-Negative Bacteria Moritella viscosa. Mar. Drugs 2020, 18, 102.

Show more citation formats Show less citations formats
Note that from the first issue of 2016, MDPI journals use article numbers instead of page numbers. See further details here.

Article Access Map by Country/Region

1
Back to TopTop