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Open AccessArticle

Quantitative and Qualitative HPLC Analysis of Mycosporine-Like Amino Acids Extracted in Distilled Water for Cosmetical Uses in Four Rhodophyta

1
Department of Ecology and Geology, Faculty of Sciences, University of Malaga, Institute of Blue Biotechnology and Development (IBYDA), Campus Universitario de Teatinos s/n, E-29071 Malaga, Spain
2
Photobiology Laboratory, Central Service for Research Support (SCAI), University of Malaga, Campus Universitario de Teatinos s/n, E-29071 Malaga, Spain
*
Author to whom correspondence should be addressed.
Mar. Drugs 2020, 18(1), 27; https://doi.org/10.3390/md18010027
Received: 28 November 2019 / Revised: 21 December 2019 / Accepted: 25 December 2019 / Published: 28 December 2019
(This article belongs to the Special Issue Mycosporine-Like Amino Acids from Marine Resource)
Mycosporine-like amino acids (MAAs) have gained considerable attention as highly active photoprotective candidates for human sunscreens. However, more studies are necessary to evaluate the extraction efficiencies of these metabolites in cosmetic compatible solvents, as well as, their subsequent HPLC analysis. In the present study, MAA extraction using distilled water and 20% aqueous methanol in four Rhodophyta was investigated. Different re-dissolution solvents and a C8 and C18 columns were tested for the HPLC analysis. Porphyra-334, shinorine, palythine, palythine-serine, asterina-330, and palythinol were identified by HPLC/ESI-MS. The separation of these MAAs were improved employing the C8-column, and using methanol as re-dissolution solvent. Regarding total MAAs concentrations, no differences between the two solvents were found. The highest MAA amounts were observed injecting them directly in the HPLC. According to these results, distilled water could be an excellent extraction solvent for MAAs. Nevertheless, the re-dissolution in pure methanol after dryness would be the best option for the qualitative analysis of the most common MAAs in these red algae. Our results entail important implications regarding the use of red macroalgae as promising candidates as environment-friendly sources of natural sunscreens. View Full-Text
Keywords: HPLC column; mycosporine-like amino acids; photoprotection; re-dissolution solvent; Rhodophyta HPLC column; mycosporine-like amino acids; photoprotection; re-dissolution solvent; Rhodophyta
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Chaves-Peña, P.; de la Coba, F.; Figueroa, F.L.; Korbee, N. Quantitative and Qualitative HPLC Analysis of Mycosporine-Like Amino Acids Extracted in Distilled Water for Cosmetical Uses in Four Rhodophyta. Mar. Drugs 2020, 18, 27.

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