A Marine Isolate of Bacillus pumilus Secretes a Pumilacidin Active against Staphylococcus aureus
AbstractProducing antimicrobials is a common adaptive behavior shared by many microorganisms, including marine bacteria. We report that SF214, a marine-isolated strain of Bacillus pumilus, produces at least two different molecules with antibacterial activity: a molecule smaller than 3 kDa active against Staphylococcus aureus and a molecule larger than 10 kDa active against Listeria monocytogenes. We focused our attention on the anti-Staphylococcus molecule and found that it was active at a wide range of pH conditions and that its secretion was dependent on the growth phase, medium, and temperature. A mass spectrometry analysis of the size-fractionated supernatant of SF214 identified the small anti-Staphylococcus molecule as a pumilacidin, a nonribosomally synthesized biosurfactant composed of a mixture of cyclic heptapeptides linked to fatty acids of variable length. The analysis of the SF214 genome revealed the presence of a gene cluster similar to the srfA-sfp locus encoding the multimodular, nonribosomal peptide synthases found in other surfactant-producing bacilli. However, the srfA-sfp cluster of SF214 differed from that present in other surfactant-producing strains of B. pumilus by the presence of an insertion element previously found only in strains of B. safensis. View Full-Text
- Supplementary File 1:
PDF-Document (PDF, 103 KB)
Share & Cite This Article
Saggese, A.; Culurciello, R.; Casillo, A.; Corsaro, M.M.; Ricca, E.; Baccigalupi, L. A Marine Isolate of Bacillus pumilus Secretes a Pumilacidin Active against Staphylococcus aureus. Mar. Drugs 2018, 16, 180.
Saggese A, Culurciello R, Casillo A, Corsaro MM, Ricca E, Baccigalupi L. A Marine Isolate of Bacillus pumilus Secretes a Pumilacidin Active against Staphylococcus aureus. Marine Drugs. 2018; 16(6):180.Chicago/Turabian Style
Saggese, Anella; Culurciello, Rosanna; Casillo, Angela; Corsaro, Maria M.; Ricca, Ezio; Baccigalupi, Loredana. 2018. "A Marine Isolate of Bacillus pumilus Secretes a Pumilacidin Active against Staphylococcus aureus." Mar. Drugs 16, no. 6: 180.
Note that from the first issue of 2016, MDPI journals use article numbers instead of page numbers. See further details here.