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Mar. Drugs 2018, 16(11), 435; https://doi.org/10.3390/md16110435

Heterologous Expression of the Nybomycin Gene Cluster from the Marine Strain Streptomyces albus subsp. chlorinus NRRL B-24108

1
Pharmazeutische Biotechnologie, Universität des Saarlandes, 66123 Saarbrücken, Germany
2
Helmholtz-Institut für Pharmazeutische Forschung Saarland, 66123 Saarbrücken, Germany
*
Author to whom correspondence should be addressed.
Received: 5 October 2018 / Revised: 26 October 2018 / Accepted: 31 October 2018 / Published: 4 November 2018
(This article belongs to the Special Issue Microbial Gene Clusters of Marine Origin)
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Abstract

Streptomycetes represent an important reservoir of active secondary metabolites with potential applications in the pharmaceutical industry. The gene clusters responsible for their production are often cryptic under laboratory growth conditions. Characterization of these clusters is therefore essential for the discovery of new microbial pharmaceutical drugs. Here, we report the identification of the previously uncharacterized nybomycin gene cluster from the marine actinomycete Streptomyces albus subsp. chlorinus through its heterologous expression. Nybomycin has previously been reported to act against quinolone-resistant Staphylococcus aureus strains harboring a mutated gyrA gene but not against those with intact gyrA. The nybomycin-resistant mutants generated from quinolone-resistant mutants have been reported to be caused by a back-mutation in the gyrA gene that restores susceptibility to quinolones. On the basis of gene function assignment from bioinformatics analysis, we suggest a model for nybomycin biosynthesis. View Full-Text
Keywords: streptomycetes; secondary metabolites; nybomycin gene cluster; heterologous expression; nybomycin biosynthesis streptomycetes; secondary metabolites; nybomycin gene cluster; heterologous expression; nybomycin biosynthesis
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Rodríguez Estévez, M.; Myronovskyi, M.; Gummerlich, N.; Nadmid, S.; Luzhetskyy, A. Heterologous Expression of the Nybomycin Gene Cluster from the Marine Strain Streptomyces albus subsp. chlorinus NRRL B-24108. Mar. Drugs 2018, 16, 435.

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