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Mar. Drugs 2017, 15(11), 332;

Determination of the Deacetylation Degree of Chitooligosaccharides

2,* and 1,*
Guangdong Engineering Research Center of Natural Products and New Drugs, Guangdong Pharmaceutical University, Guangzhou 510006, China
Guangdong Metabolic Diseases Research Center of Integrated Chinese and Western Medicine, Key Unit of Modulating Liver to Treat Hyperlipemia SATCM (State Administration of Traditional Chinese Medicine), Guangdong Pharmaceutical University, Guangzhou 510006, China
Guangdong Food and Drug Vocational Technical School, Guangzhou 510663, China
Shenzhen Center for Disease Control and Prevention, 8 Longyuan Road, Nanshan District, Shenzhen 518055, China
These authors have equally contributed to this work and should be considered co-first authors.
Authors to whom correspondence should be addressed.
Received: 25 August 2017 / Revised: 12 October 2017 / Accepted: 23 October 2017 / Published: 25 October 2017
(This article belongs to the Special Issue Marine Chitin)
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The methods for determination of chitosan content recommended in the Chinese Pharmacopoeia and the European Pharmacopoeia are not applicable for evaluation of the extent of deacetylation (deacetylation degree, DD) in chitooligosaccharides (COS). This study explores two different methods for assessment of DD in COS having relatively high and low molecular weights: an acid-base titration with bromocresol green indicator and a first order derivative UV spectrophotometric method for assessment of DD in COS. The accuracy of both methods as a function of molecular weight was also investigated and compared to results obtained using 1H NMR spectroscopy. Our study demonstrates two simple, fast, widely adaptable, highly precise, accurate, and inexpensive methods for the effective determination of DD in COS, which have the potential for widespread commercial applications in developing country. View Full-Text
Keywords: chitooligosaccharides; deacetylation; bromocresol green; acid-base titration; first-order derivative UV spectra; 1H NMR chitooligosaccharides; deacetylation; bromocresol green; acid-base titration; first-order derivative UV spectra; 1H NMR

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This is an open access article distributed under the Creative Commons Attribution License which permits unrestricted use, distribution, and reproduction in any medium, provided the original work is properly cited (CC BY 4.0).

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Jiang, Y.; Fu, C.; Wu, S.; Liu, G.; Guo, J.; Su, Z. Determination of the Deacetylation Degree of Chitooligosaccharides. Mar. Drugs 2017, 15, 332.

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