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Open AccessArticle

Preliminary Characterization, Antioxidant Properties and Production of Chrysolaminarin from Marine Diatom Odontella aurita

by Song Xia 1,2,†, Baoyan Gao 1,†, Aifen Li 1, Jihai Xiong 2, Ziqiang Ao 2 and Chengwu Zhang 1,*
1
Institute of Hydrobiology, Jinan University, Guangzhou 510632, China
2
Institute of Energy Research, Jiangxi Academy of Sciences, Nanchang 330096, China
*
Author to whom correspondence should be addressed.
These authors contributed equally to this work.
Mar. Drugs 2014, 12(9), 4883-4897; https://doi.org/10.3390/md12094883
Received: 26 June 2014 / Revised: 9 September 2014 / Accepted: 9 September 2014 / Published: 23 September 2014
(This article belongs to the Collection Marine Polysaccharides)
A new chrysolaminarin, named CL2, with a molecular mass of 7.75 kDa, was purified from the marine diatom, Odontella aurita, using DEAE-52 cellulose anion-exchange chromatography and Sephadex G-200 gel-filtration chromatography. The monosaccharide and structural analysis revealed that CL2 was a glucan mainly composed of glucose, which was linked by the β-d-(1→3) (main chain) and β-d-(1→6) (side chain) glycosidic bond, demonstrated by infrared spectroscopy (IR) and nuclear magnetic resonance (NMR). The antioxidant activity tests revealed that the CL2 presented stronger hydroxyl radical scavenging activity with increasing concentrations, but less was effective on reducing power analysis and scavenging 1,1-diphenyl-2-picrylhydrazyl (DPPH) radical. The influences of nitrogen concentration and light intensity on chrysolaminarin production of O. aurita were further investigated in a glass column photobioreactor, and a record high chrysolaminarin productivity of 306 mg L−1 day−1 was achieved. In conclusion, the chrysolaminarin CL2 from O. aurita may be explored as a natural antioxidant agent for application in aquaculture, food and pharmaceutical areas. View Full-Text
Keywords: Odontella aurita; chrysolaminarin; structural characteristics; antioxidant activity; productivity Odontella aurita; chrysolaminarin; structural characteristics; antioxidant activity; productivity
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Xia, S.; Gao, B.; Li, A.; Xiong, J.; Ao, Z.; Zhang, C. Preliminary Characterization, Antioxidant Properties and Production of Chrysolaminarin from Marine Diatom Odontella aurita. Mar. Drugs 2014, 12, 4883-4897.

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