High-Resolution Melting-Based Quantitative Analysis of RASSF1 Methylation in Breast Cancer
Abstract
Material and Methods. A total of 76 breast carcinomas and 10 noncancerous breast tissues were studied by means of HRM and compared with the results obtained by means of quantitative methylation-specific polymerase chain reaction (QMSP) and methylation-specific polymerase chain reaction (MSP).
Results. Both quantitative methods, HRM and QMSP, showed a similar specificity and sensitivity for the detection of RASSF1 methylation in breast cancer (about 80% and 70%, respectively). In breast cancer, the mean methylation intensity of RASSF1 was 42.5% and 48.6% according to HRM and QMSP, respectively. Both methods detected low levels of methylation (less than 5%) in noncancerous breast tissues. In comparison with quantitative methods, MSP showed a lower sensitivity (70%), but a higher specificity (80%) for the detection of RASSF1 methylation in breast cancer.
Conclusions. HRM is as a simple, cost-effective method for the reliable high-throughput quantification of DNA methylation in clinical material.
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Stuopelytė, K.; Daniūnaitė, K.; Laurinavičienė, A.; Ostapenko, V.; Jarmalaitė, S. High-Resolution Melting-Based Quantitative Analysis of RASSF1 Methylation in Breast Cancer. Medicina 2013, 49, 14. https://doi.org/10.3390/medicina49020014
Stuopelytė K, Daniūnaitė K, Laurinavičienė A, Ostapenko V, Jarmalaitė S. High-Resolution Melting-Based Quantitative Analysis of RASSF1 Methylation in Breast Cancer. Medicina. 2013; 49(2):14. https://doi.org/10.3390/medicina49020014
Chicago/Turabian StyleStuopelytė, Kristina, Kristina Daniūnaitė, Aida Laurinavičienė, Valerijus Ostapenko, and Sonata Jarmalaitė. 2013. "High-Resolution Melting-Based Quantitative Analysis of RASSF1 Methylation in Breast Cancer" Medicina 49, no. 2: 14. https://doi.org/10.3390/medicina49020014