Human Herpesvirus 6 and 7 Reactivation and Disease Activity in Multiple Sclerosis
by
Zaiga Nora-Krukle
1,*, 
Svetlana Chapenko
1,
Inara Logina
2,
Andrejs Millers
2,
Ardis Platkajis
3 and
Modra Murovska
1 1
August Kirchenstein Institute of Microbiology and Virology, Riga Stradins University
2
Department of Neurology and Neurosurgery, Riga Stradins University
3
Department of Radiology, Riga Stradins University, Latvia
*
Author to whom correspondence should be addressed.
Medicina 2011, 47(10), 75; https://doi.org/10.3390/medicina47100075
Submission received: 13 September 2011
/
Accepted: 31 October 2011
/
Published: 5 November 2011
Abstract
Recent studies have focused on the associations between human herpesvirus 6 (HHV-6) and human herpesvirus 7 (HHV-7), and multiple sclerosis (MS). The aim of this study was to investigate the associations between HHV-6 and HHV-7 reactivation and MS disease activity, and interleukin 12 (IL-12) and tumor necrosis factor α (TNF-α) production.
Material and Methods. The frequency of plasma viremia by nested polymerase chain reaction and transcription of viral mRNA in peripheral blood mononuclear cells by reverse transcriptasepolymerase chain reaction (RT-PCR) of 14 relapsing/remitting (RR) and 14 secondary progressive (SP) MS patients were studied in comparison with clinical manifestation of the disease. Serum concentrations of cytokines IL-12 and TNF-α were analyzed by enzyme-linked immunosorbent assay.
Results. Plasma samples from 25 of the 28 MS patients with estimated latent/persistent HHV-6 and/or HHV-7 infection were examined during relapse and remission/relative remission. HHV-6 reactivation was found in 4 of the 7 RRMS and 4 of the 7 SPMS patients, and HHV-7 reactivation was identified in 3 of the 7 RRMS and 1 of the 7 SPMS patients (all in relapse). In 2 of the 3 RRMS patients without viremia in relapse, HHV-6 mRNA transcription was detected. In RRMS and SPMS patients with active HHV-6 and HHV-7 infection in relapse, the serum concentrations of IL-12 and TNF-α were significantly higher than in those with latent virus infection.
Conclusions. HHV-6 and HHV-7 reactivation could be implicated in the exacerbation of MS via activation of Th1 lymphocyte subsets.
Material and Methods. The frequency of plasma viremia by nested polymerase chain reaction and transcription of viral mRNA in peripheral blood mononuclear cells by reverse transcriptasepolymerase chain reaction (RT-PCR) of 14 relapsing/remitting (RR) and 14 secondary progressive (SP) MS patients were studied in comparison with clinical manifestation of the disease. Serum concentrations of cytokines IL-12 and TNF-α were analyzed by enzyme-linked immunosorbent assay.
Results. Plasma samples from 25 of the 28 MS patients with estimated latent/persistent HHV-6 and/or HHV-7 infection were examined during relapse and remission/relative remission. HHV-6 reactivation was found in 4 of the 7 RRMS and 4 of the 7 SPMS patients, and HHV-7 reactivation was identified in 3 of the 7 RRMS and 1 of the 7 SPMS patients (all in relapse). In 2 of the 3 RRMS patients without viremia in relapse, HHV-6 mRNA transcription was detected. In RRMS and SPMS patients with active HHV-6 and HHV-7 infection in relapse, the serum concentrations of IL-12 and TNF-α were significantly higher than in those with latent virus infection.
Conclusions. HHV-6 and HHV-7 reactivation could be implicated in the exacerbation of MS via activation of Th1 lymphocyte subsets.
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MDPI and ACS Style
Nora-Krukle, Z.; Chapenko, S.; Logina, I.; Millers, A.; Platkajis, A.; Murovska, M. Human Herpesvirus 6 and 7 Reactivation and Disease Activity in Multiple Sclerosis. Medicina 2011, 47, 75. https://doi.org/10.3390/medicina47100075
AMA Style
Nora-Krukle Z, Chapenko S, Logina I, Millers A, Platkajis A, Murovska M. Human Herpesvirus 6 and 7 Reactivation and Disease Activity in Multiple Sclerosis. Medicina. 2011; 47(10):75. https://doi.org/10.3390/medicina47100075
Chicago/Turabian StyleNora-Krukle, Zaiga, Svetlana Chapenko, Inara Logina, Andrejs Millers, Ardis Platkajis, and Modra Murovska. 2011. "Human Herpesvirus 6 and 7 Reactivation and Disease Activity in Multiple Sclerosis" Medicina 47, no. 10: 75. https://doi.org/10.3390/medicina47100075
